PXD058952 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Metabolic Trajectories in Human Neocortical Development |
| Description | The shift from glycolysis to oxidative phosphorylation is a key step in neural differentiation. Yet, the timing of metabolic rewiring in the development from progenitors to neurons remains elusive, especially in human corticogenesis. To bridge this gap, here we adopted a unique cell platform based on neocortex-derived human neuroepithelial stem cells, modeling their physiological transition into functional neurons in a four-month longitudinal study. Using quantitative proteomics, combined with NAD(P)H fluorescence lifetime imaging in living cells, we investigated the entire differentiation process and described how metabolic pathways drive cortical neuron maturation. The prevalent metabolic adaptations were then confirmed in cerebro-cortical organoids and in a human neocortical specimen. This study provides a spatio-temporal map of neocortical metabolism during development and offers a framework to investigate human neurometabolic disorders. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-18 |
| AnnouncementXML | Submission_2026-03-18_13:52:44.090.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Francesco Greco |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-12-17 14:19:27 | ID requested | |
| ⏵ 1 | 2026-03-18 13:52:50 | announced | |
Publication List
Keyword List
| submitter keyword: neurons, neocortex,metabolism, neuroepithelial stem cells |
Contact List
| Liam Andrew McDonnell |
|---|
| contact affiliation | Fondazione Pisana per la Scienza, ONLUS, San Giuliano Terme, Pisa, Italy |
| contact email | liam@fpscience.it |
| lab head | |
| Francesco Greco |
|---|
| contact affiliation | Fondazione Toscana Gabriele Monasterio |
| contact email | f.greco@fpscience.it |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD058952 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058952
- Label: PRIDE project
- Name: Metabolic Trajectories in Human Neocortical Development
