PXD058950-1

PXD058950 is an original dataset announced via ProteomeXchange.

Title	Defining the molecular impacts of Humalite application on field-grown wheat (Triticum aestivum L.) using quantitative proteomics
Description	Increasing global food production demands have resulted in increased fertilizer usage, causing detrimental environmental impacts. Biostimulants, such as humic substances, are currently being applied as a strategy to increase plant nutrient-use efficiency and minimize environmental impacts within cropping systems. Humalite is a unique, naturally occurring coal-like substance found in deposits across southern Alberta. These deposits contain exceptionally high ratios of humic acids (>70%) and micronutrients due to their unique freshwater depositional environment. Humalite has begun to be applied to fields by local growers in Alberta, despite limited scientific data on yield impacts across diverse crops. Recent work has shown positive impacts on plant growth, yield and nutrient usage in wheat plants supplemented with Humalite; however, there is little known of the impact of Humalite at the molecular level. Here, we report a quantitative proteomics approach to identify systems-level molecular changes induced by the addition of different Humalite application rates in field-grown wheat (Triticum aestivum L.) under three urea fertilizer application rates. In particular, we see wide-ranging protein abundance changes in proteins associated with several metabolic pathways and growth-related biological processes that suggest how Humalite modulates the plant molecular landscape. Our results provide new, functional information that will help better inform agricultural producers on optimal biostimulant and fertilizer usage.
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-27_16:37:42.873.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Uhrig
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-17 11:29:54	ID requested
⏵ 1	2025-07-27 16:37:43	announced

10.1002/pmic.13981;

Grubb LE, Talasila M, Gorim LY, Uhrig RG, Defining the Molecular Impacts of Humalite Application on Field-Grown Wheat (Triticum aestivum L.) Using Quantitative Proteomics. Proteomics, 25(14):e13981(2025) [pubmed]

submitter keyword: Humalite, Proteomics,Wheat

R. Glen Uhrig
contact affiliation	Department of Biological Sciences, University of Alberta
contact email	ruhrig@ualberta.ca
lab head
Richard Uhrig
contact affiliation	University of Alberta
contact email	ruhrig@ualberta.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD058950

PRIDE project URI

• PRIDE
  1. PXD058950
     1. Label: PRIDE project
     2. Name: Defining the molecular impacts of Humalite application on field-grown wheat (Triticum aestivum L.) using quantitative proteomics