PXD058942-1

PXD058942 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of chlorophyll synthase-associated auxiliary proteins in Synechocystis photosystem II biogenesis using a FLAG-tag and 15N-labelled internal standards.
Description	Photosystem II (PSII) is embedded in the thylakoid membranes of cyanobacteria and plant chloroplasts. PSII is the component of the photosynthetic apparatus that uses solar energy to split water, producing the oxygen in Earth’s atmosphere. PSII is a multi-subunit protein complex that requires several auxiliary proteins to manage its assembly in the thylakoid membrane which also includes the insertion of chlorophyll into its structure. One of these auxiliary proteins Ycf39 associates with two 5-6 kDa chlorophyll-binding proteins HliC and HliD, forming a complex which is involved in the insertion of chlorophyll into the D1 subunit of PSII. In addition, an earlier study (Chidgey et al. (2014) Plant Cell 26: 1267-79) has shown that these 3 proteins can associate with chlorophyll synthase (ChlG), the enzyme responsible for catalysing the final step of chlorophyll biosynthesis, the attachment of the C20 phytyl group to the Mg-containing tetrapyrrole ring. Using FLAG-tagged ChlG to capture Ycf39 and associated proteins and 15N-labelled artificial protein standards to enable protein quantification, we have characterized the effects of both normal and high illumination, together with an HliC knock-out mutant on the ChlG-Ycf39-HliC/D complex.
HostingRepository	PRIDE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-15_16:10:53.104.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Philip Jackson
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-17 06:22:15	ID requested
⏵ 1	2025-06-15 16:10:53	announced

10.1093/plphys/kiaf213;

Wysocka A, Kulik N, Shukla MK, Opat, í, kov, á M, Kou, ř, il R, Jackson PJ, Brindley AA, Janou, š, kovec J, Kiss É, Hitchcock A, Komenda J, Hunter CN, Sobotka R, High-light-inducible proteins control associations between chlorophyll synthase and the Photosystem II biogenesis factor Ycf39. Plant Physiol, 198(2):(2025) [pubmed]

submitter keyword: biogenesis, ChlG, photosystem II, thylakoid,Synechocystis, HliD, FLAG-tag, HliC, chlorophyll synthase, Ycf39

Professor Roman Sobotka
contact affiliation	Centre Algatech, Institute of Microbiology, Academy of Sciences of the Czech Republic, Třeboň,CZ.
contact email	sobotka@alga.cz
lab head
Philip Jackson
contact affiliation	School of Biosciences, University of Sheffield
contact email	p.j.jackson@sheffield.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD058942

PRIDE project URI

• PRIDE
  1. PXD058942
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of chlorophyll synthase-associated auxiliary proteins in Synechocystis photosystem II biogenesis using a FLAG-tag and 15N-labelled internal standards.