PXD058934 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Differentiation of wheat high-molecular-weight glutenin subunits |
| Description | High-molecular-weight glutenin subunits (HMW-GS) play an essential role in the end-use quality of wheat (Triticum aestivum L.). We developed a targeted liquid chromatography-tandem mass spectrometry method in parallel reaction monitoring (LC-MS/MS-PRM) to detect and differentiate wheat HMW-GS in German wheat cultivars with known (37 cultivars) and unknown (58 cultivars) composition. The newly development method is suitable to unambiguously identify Ax1, Ax2*, Bx6, Bx14, Dx2, Dx5, Dy10, Dy12, as well as no expression of Ax, but cannot distinguish Bx7 and Bx17 and identify the variation of By due to high sequence identity to Dy and within By. The method is further suited to clearly conclude, if the sample is a mixture of at least two cultivars or consists of only one cultivar. In comparison to gel-based methods (SDS-PAGE), UV-detection after LC (RP-HPLC-UV) and MS of intact proteins (MALDI-TOF-MS), LC-MS/MS has a high resolution, is less biased by interpretation and provides more insights on molecular level, especially, if the peptides are not only analyzed by LC-MS/MS-PRM, but also by untargeted LC-MS/MS. The used procedure can be applied to expand the LC-MS/MS-PRM method for more HMW-GS or even to other wheat proteins, e.g., low-molecular-weight glutenin subunits (LMW-GS), in future. This study describes the first MS-based method on peptide level for the differentiation of wheat HMW-GS. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-10 |
| AnnouncementXML | Submission_2026-09-10_07:22:32.359.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sabrina Geisslitz |
| SpeciesList | scientific name: Triticum aestivum (Wheat); NCBI TaxID: NEWT:4565; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-12-17 01:02:43 | ID requested | |
| ⏵ 1 | 2026-09-10 07:22:32 | announced | |
Publication List
Keyword List
| submitter keyword: HMW-GS |
| Triticum aestivum L. |
| wheat proteomics |
| untargeted nLC-MS/MS |
| parallel reaction monitoring |
Contact List
| Antoine America |
|---|
| contact affiliation | Wageningen Plant Research, Wageningen University & Research, Wageningen, The Netherlands |
| contact email | twan.america@wur.nl |
| lab head | |
| Sabrina Geisslitz |
|---|
| contact affiliation | Leibniz Institute for Food Systems Biologoy at the Technical University of Munich |
| contact email | s.geisslitz.leibniz-lsb@tum.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058934
- Label: PRIDE project
- Name: Differentiation of wheat high-molecular-weight glutenin subunits
