PXD058915-1

PXD058915 is an original dataset announced via ProteomeXchange.

Title	Canonical and isomiR products of miR-142 enhance dendritic cell reprogramming
Description	MicroRNAs (miRNAs) regulate gene expression and generate isoforms (isomiRs) with distinct targeting properties, but their roles in cellular specification and reprogramming remain poorly understood. Here, we investigated miRNAs and isomiRs during direct reprogramming to conventional type 1 dendritic cells (cDC1s), identifying miR-124 and miR-142 as facilitators of the process. The canonical miR-124-3p strand induced a permissive chromatin landscape enriched for cooperative transcription factor motifs, thereby transiently increasing reprogramming efficiency. In contrast, miR-142 enhanced lineage fidelity by repressing fibroblast identity and activating cDC1-specific programs through cooperating isomiRs that drove XCR1 expression and increased type III interferon production. In addition, miR-142 mimics enhanced cancer cell reprogramming and anti-tumor immunity in vivo. Finally, we developed RNA-based cDC1 reprogramming using transcription factor mRNAs combined with miRNA mimics. Together, these findings uncover miRNA isoform diversity as a programmable regulatory layer in immune cell specification and establish RNA-driven reprogramming as a non-viral strategy for cancer immunotherapy.
HostingRepository	PRIDE
AnnounceDate	2026-07-16
AnnouncementXML	Submission_2026-07-16_04:16:35.598.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sudip Ghosh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-16 17:08:51	ID requested
⏵ 1	2026-07-16 04:16:36	announced

Dataset with its publication pending

submitter keyword: Reprogramming, miRNA, data-independent acquisition, dendritic cells

Dr. Jenny Hansson
contact affiliation	Proteomic Hematology Department of Experimental Medical Science Lund Stem Cell Center, Lund University
contact email	jenny.hansson@med.lu.se
lab head
Sudip Ghosh
contact affiliation	Proteomic Hematology, Department of Experimental Medical Science, Lund Stem Cell Center, Lund University
contact email	sudip.ghosh@med.lu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058915
     1. Label: PRIDE project
     2. Name: Canonical and isomiR products of miR-142 enhance dendritic cell reprogramming