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PXD058877-1

PXD058877 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of intramolecular disulfide bonds in GrxS12
DescriptionTo investigate whether the conserved Cys residues of Grxs form intramolecular or intermolecular disulfide bonds, the recombinant GrxS12 proteins were purified, and subsequently separated using non-reducing SDS-PAGE. The gel revealed the presence of a monomer band but not a dimer band, indicating the absence of intermolecular disulfide bonds. The protein from the monomer band was subjected to proteolytic digestion and subsequent analysis by spectrometry. The spectrometric data revealed that the GrxS12 formed an intramolecular disulfide linkage between the T31WJC34SYSSQVK41 and H88IGGC92SDTLQLHNKGELEAILAEANGK114 peptides.
HostingRepositoryPRIDE
AnnounceDate2026-01-16
AnnouncementXMLSubmission_2026-01-15_17:57:50.240.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYanshuang Liu
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationListdihydroxylated residue; monohydroxylated residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-12-15 00:54:34ID requested
12026-01-15 17:57:50announced
Publication List
10.1111/TPJ.70666;
Keyword List
submitter keyword: GrxS12, identification, intramolecular disulfide bond
Contact List
Shaojun Dai
contact affiliationShanghai Normal University,Shanghai,China
contact emaildaishaojun@shnu.edu.cn
lab head
Yanshuang Liu
contact affiliationShanghai Normal University
contact email920662600@qq.com
dataset submitter
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Dataset FTP location
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