PXD058818-1

PXD058818 is an original dataset announced via ProteomeXchange.

Title	Temporal dynamics of the interstitial fluid proteome in human skeletal muscle following exhaustive exercise
Description	The skeletal muscle interstitial space is the extracellular region around myofibres and mediates crosstalk between resident cell types. We applied a proteomic workflow to characterise the human skeletal muscle interstitial fluid proteome at rest and in response to exercise. Following exhaustive exercise, markers of skeletal muscle damage accumulate in the interstitial space followed in turn by the appearance of immune cell-derived proteins. Among the proteins upregulated after exercise, we identified cathelicidin-related antimicrobial peptide (CAMP) as a bioactive molecule regulating muscle fibre development. Treatment with the bioactive peptide derivative of CAMP (LL-37) resulted in the growth of larger C2C12 skeletal muscle myotubes. Phosphoproteomics revealed that LL-37 activated pathways central to muscle growth and proliferation, including PI3K, AKT, MAPKs, and mTOR. Our findings provide a proof-of-concept that the interstitial fluid proteome can be quantified via microdialysis sampling in vivo. Furthermore, these data highlight the importance of cellular communication in the adaptive response to exercise.
HostingRepository	PRIDE
AnnounceDate	2025-01-16
AnnouncementXML	Submission_2025-01-16_03:39:11.408.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ben Stocks
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	timsTOF Pro 2; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-12 09:42:44	ID requested
⏵ 1	2025-01-16 03:39:11	announced

10.1126/SCIADV.ADP8608;

submitter keyword: Secretomics, exercise, interstitial fluid,Skeletal muscle

Atul S Deshmukh
contact affiliation	Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen
contact email	atuldeshmukh@sund.ku.dk
lab head
Ben Stocks
contact affiliation	University of Copenhagen
contact email	ben.stocks@sund.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058818
     1. Label: PRIDE project
     2. Name: Temporal dynamics of the interstitial fluid proteome in human skeletal muscle following exhaustive exercise