PXD058797-1

PXD058797 is an original dataset announced via ProteomeXchange.

Title	Affinity peptide ligands: New tools for chasing non-canonical N-phosphoproteome
Description	Protein N-phosphorylation enrichment is faced with great difficulties due to the intrinsic instability of the N-P bond, which has seriously hindered its biological function unraveling. Current methods for N-phosphopeptides enrichment are challenging to fulfil high selectivity for all types of N-phosphorylation with minimum contamination. To address this, we reported the first affinity peptide functionalized magnetic beads for N-phosphopeptides enrichment under neutral aqueous solution. The affinity peptide, capable of binding the N-PO3 group specifically, was identified using phage display technology based on a delicate target. We demonstrated robust enrichment capacity of the peptide-modified magnetic beads across diverse biological samples, from prokaryotes to eukaryotes, as well as various organelles and tissues. Accordingly, 262 and 2307 N-phosphorylation sites were identified from E.coli and HeLa cells, which demonstrated the enhanced enrichment capability and greatly expanded the scale of N-phosphoproteome databases. Furthermore, we found that N-phosphorylation was highly enriched in the nucleus and played a vital role in regulating processes like DNA repair. Given the high efficiency and quantitative reproducibility of the method, it was found that N-phosphorylation level for kinases and proteins involved in regulation of metabolism and microtubules varied during the progression of Alzheimer's disease. Our work expands the tools library for N-phosphorylated proteome, and provides critical insights into the roles of N-phosphorylated proteins in physiology and pathology processes.
HostingRepository	iProX
AnnounceDate	2024-12-12
AnnouncementXML	Submission_2024-12-11_20:35:23.936.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	He Wang
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-11 20:34:40	ID requested
⏵ 1	2024-12-11 20:35:24	announced

Dataset with its publication pending

submitter keyword: Phage Display Technology, Affinity Peptide, N-phosphopeptides Enrichment , Neutral Aqueous Solution (or organelle) ,Quantitation Analysis

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	lihuazhang@dicp.ac.cn
lab head
He Wang
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	crane_wanghe@dicp.ac.cn
dataset submitter

iProX dataset URI