PXD058726-1

PXD058726 is an original dataset announced via ProteomeXchange.

Title	Membrane IL-18 Identifies a Unique Human Macrophage Subset with Distinct Phenotypic and Functional Properties
Description	This study adds a tile to the macrophage heterogeneity in humans. We characterize a unique macrophage population expressing membrane-associated IL-18 (mIL-18) that shows peculiar proteomic, phenotypic, ultrastructural, and functional properties. mIL-18+ macrophages exhibit increased levels of key proteins for pathogen recognition, activation, migration, and endocytosis. They also display specialized functions in vesicle and actin filament transport, lipid metabolism, and have typical mitochondrial traits. mIL-18 identifies most macrophages in the peritoneum of adult cancer patients, where it is co-expressed with TREM2. Conversely, two populations of TREM2+ cells, either expressing or lacking mIL-18, colonize the bone marrow of pediatric patients with neuroblastoma, Importantly, in primary neuroblastomas, transcriptional signatures associated with mIL-18 expression show different prognostic values. These data indicate a potentiated role for the mIL-18+ macrophages in the surveillance of tumor- and inflammatory-associated microenvironments.
HostingRepository	PRIDE
AnnounceDate	2025-11-17
AnnouncementXML	Submission_2025-11-16_16:41:21.868.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Bartolucci
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-10 02:44:29	ID requested
⏵ 1	2025-11-16 16:41:22	announced

10.1080/2162402x.2025.2571774;

Vitale C, Petretto A, Cortese K, Carta S, Dondero A, Lavarello C, Cangelosi D, Morini M, Bellora F, Arnaldi P, Loiacono F, Bruzzone S, Piacente F, Bruno S, Serra M, Pessino A, Mammoliti S, Garaventa A, Conte M, Locati M, Norata GD, Colonna M, Vivier E, Bottino C, Castriconi R, Membrane IL-18 identifies a human macrophage subset with distinct proteomic and functional traits. Oncoimmunology, 14(1):2571774(2025) [pubmed]

submitter keyword: IL-18,Macrophages, adult cancer patients

pediatric neuroblastoma, endocytosis, TREM2

Andrea Petretto
contact affiliation	IRCCS Istituto G Gaslini
contact email	a.petretto@gmail.com
lab head
Martina Bartolucci
contact affiliation	IRCCS Gaslini
contact email	smartibartolucci@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD058726

PRIDE project URI

• PRIDE
  1. PXD058726
     1. Label: PRIDE project
     2. Name: Membrane IL-18 Identifies a Unique Human Macrophage Subset with Distinct Phenotypic and Functional Properties