PXD058700-1

PXD058700 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling on sex-specific changes on skeletal muscle after exercise
Description	Biological sex-based differences are reported for aerobic capacity, exercise performance, skeletal muscle mass and fiber-type composition. We aimed to provide a yet missing comprehensive picture of molecular differences between female and male skeletal muscle before and during an 8-week exercise intervention. We characterized muscle biopsies from 25 (16f/9m) subjects with overweight and obesity in a multi-omics approach employing epigenomics, transcriptomics and proteomics at baseline, after acute exercise and after 8 weeks of supervised endurance training. Further we investigated which differences were conserved in myotubes obtained from these donors or can be restored by sex hormone treatment. We found differential CpG-site methylation in 16.012 genes, 1.366 differentially expressed genes and 120 differentially abundant proteins at baseline. Only non-autosomal gene expression was conserved in vitro. Part of sex-specific transcriptomic differences in vivo could be mimicked by sex hormone treatment in vitro. Differences in the proteome were dominated by higher abundance of proteins regulating glycogen degradation, glycolysis and of other fast-twitch fiber type proteins in males. Females showed higher abundance of proteins regulating fatty acid handling. The initial differences in fiber type-specific proteins were diminished after 8-week training, and both sexes adapted to endurance training by upregulating mitochondrial proteins involved in substrate oxidation and ATP production. Acute exercise upregulated stress-responsive transcripts predominantly in males. In conclusion, the sex-specific composition of the proteome underpins the differences in glucose and fatty acid metabolism in female and male skeletal muscle. Training mitigates these differences toward an endurance-trained proteomic profile in both sexes after only 8 weeks.
HostingRepository	PRIDE
AnnounceDate	2025-09-02
AnnouncementXML	Submission_2025-09-02_14:07:20.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christine von Toerne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-09 10:52:52	ID requested
⏵ 1	2025-09-02 14:07:20	announced

10.1016/J.MOLMET.2025.102185;

submitter keyword: sex, skeletal muscle,LC-MSMS

Cora Weigert
contact affiliation	Institute for Clinical Chemistry and Pathobiochemistry, Department for Diagnostic Laboratory Medicine, University Hospital Tübingen, Hoppe-Seyler-Str. 3 72076 Tübingen, Germany
contact email	cora.weigert@med.uni-tuebingen.de
lab head
Christine von Toerne
contact affiliation	Helmholtz Zentrum München
contact email	vontoerne@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD058700

PRIDE project URI

• PRIDE
  1. PXD058700
     1. Label: PRIDE project
     2. Name: Proteomic profiling on sex-specific changes on skeletal muscle after exercise