PXD058664-1

PXD058664 is an original dataset announced via ProteomeXchange.

Title	Drug sensitivity, gene dependency and proteogenomic correlates of the spectrum of telomere maintenance mechanisms in cancer cells
Description	Replicative immortality is a hallmark of cancer, driven by activation of telomere maintenance mechanisms (TMM), that is yet to be therapeutically exploited. To expedite discoveries that will enable development of TMM-targeted therapeutics, we have generated a resource of telomere biology metrics for a pan-cancer panel of 976 cell lines. We have also produced new proteomic data from data-independent mass spectrometry for most of these cell lines. These data link to pre-existing multi-omic data, drug sensitivity and molecular dependency data from CRISPR/Cas9 knock-out screens for most cell lines. The TMM data illustrate a previously unappreciated range and heterogeneity in telomere biology measures, including telomere biology states that diverge from the binary model of TMM activation involving either telomerase or Alternative Lengthening of Telomeres (ALT). The multi-omic data were applied in conjunction with the TMM data to derive proteomic and transcriptomic predictors of ALT and telomerase activity levels. Our investigations also revealed molecular vulnerabilities of ALT cancer cells and identified an investigational drug, Pevonedistat, with activity that strongly correlates with telomerase activity levels. These discoveries illustrate the potential for leveraging this new resource of telomere biology metrics to realise the potential for TMM-directed cancer therapeutics and companion diagnostics.
HostingRepository	PRIDE
AnnounceDate	2025-10-20
AnnouncementXML	Submission_2025-10-20_14:28:40.475.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zainab Noor
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-07 07:03:40	ID requested
⏵ 1	2025-10-20 14:28:41	announced

Dataset with its publication pending

submitter keyword: TMM, Cancer, Proteomics, SWATH-DIA, Telomere biology

Karen L. MacKenzie
contact affiliation	Cancer Research Unit, Children’s Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead NSW Australia
contact email	kmackenzie@cmri.org.au
lab head
Zainab Noor
contact affiliation	ProCan, Children's Medical Research Institute, Faculty of Medicine and Health, The University of Sydney, Westmead, New South Wales, Australia
contact email	znoor@cmri.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD058664

PRIDE project URI

• PRIDE
  1. PXD058664
     1. Label: PRIDE project
     2. Name: Drug sensitivity, gene dependency and proteogenomic correlates of the spectrum of telomere maintenance mechanisms in cancer cells