PXD058649-1

PXD058649 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics Reveals the Mechanism of Alicyclobacillus acidoterrestris in Response to Acid Stress
Description	The spores of A. acidoterrestris cryopreserved at -80 ℃ were activated by inoculating in AAM medium and cultured at 45 ℃ to logarithmic phase [19]. The activated bacterial suspension was centrifuged and resuspended into pH 2.0, 2.5, 3.0, and 4.0 (Control) medium, which were correspondingly cultured in medium for 20 and 60 minutes, respectively. Scanning electron microscopy was used to monitor the bacterial activity and morphology different acid stress.Alicyclobacillus acidoterrestris (A. acidoterrestris) causes pasteurized acidic juice spoilage, resulting in a significant decline of juice quality, and causing economic losses. Exploration of A. acidoterrestris in response to acid stress could help control contamination caused by the bacteria. In this study, the mechanism of A. acidoterrestris in response to acid stress was studied by quantitative phosphoproteomics technique. Result showed that the phosphorylation of 40 proteins in A. acidoterrestris were closely related to the regulation of acid stress. The KEGG pathway enrichment analysis showed that the quorum sensing pathway which might involved in the perception of A. acidoterrestris was mainly enriched. we found that the up-regulation of Spo0A and YidC phosphorylation, which may resist acid stress by forming spores. The phosphorylation level of pyruvate kinase increased, which may improve bacterial acid stress resistance through formation of energy supply. The phosphorylation level of ABC transporter permease was significantly up-regulated, which may be part of the cell adaptation adjustment and contribute to the survival of A. acidoterrestris under acid stress. In summary, the molecular mechanism of acid stress regulation of A. acidoterrestris was proposed via quantitative phosphoproteomics, which provided a theoretical and experimental basis for further investigation acid resistance mechanism of A. acidoterrestris.
HostingRepository	PRIDE
AnnounceDate	2025-01-18
AnnouncementXML	Submission_2025-01-17_18:25:53.538.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	lingxia Jiao
SpeciesList	scientific name: Alicyclobacillus acidoterrestris; NCBI TaxID: 1450;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-06 06:39:19	ID requested
⏵ 1	2025-01-17 18:25:53	announced

Dataset with its publication pending

submitter keyword: Alicyclobacillus acidoterrestris

Acid stress

Phosphorylated proteomics

Acid tolerance mechanism

Protein modification

Jiao lingxia
contact affiliation	Henan Institute of Science and Technology, Xinxiang, Henan,
contact email	jiaolingxia@163.com
lab head
lingxia Jiao
contact affiliation	Henan Institute of Science and Technology
contact email	jiaolingxia@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058649
     1. Label: PRIDE project
     2. Name: Phosphoproteomics Reveals the Mechanism of Alicyclobacillus acidoterrestris in Response to Acid Stress