PXD058631-1

PXD058631 is an original dataset announced via ProteomeXchange.

Title	Pre-established ATF4 occupancy and chromatin organization instruct selective transcription activation during integrated stress response
Description	Cells rapidly and extensively remodel their transcriptome in response to stress to restore homeostasis, but the underlying mechanisms are not fully understood. Here we characterize the dynamic changes in transcriptome, epigenetics, and 3D genome organization during the integrated stress response (ISR). ISR induction triggers widespread transcriptional changes within 6 hours, coinciding with increased binding of ATF4, a key transcriptional effector. Notably, ATF4 binds to hundreds of genes even under non-stress conditions, priming them for stronger activation upon stress. The transcriptional changes during ISR do not rely on increased H3K27 acetylation, chromatin accessibility, or rewired enhancer-promoter looping. Instead, ATF4-mediated gene activation is linked to the redistribution of CEBPγ from non-ATF4 sites to a subset of ATF4-bound regions, likely by forming an ATF4/CEBPγ heterodimer. CEBPγ preferentially targets the sites pre-occupied by ATF4, as well as genomic regions exhibiting a unique higher-order chromatin structure signature. Thus, the transcriptional responses during ISR are largely pre-wired by intrinsic chromatin properties. These findings provide novel insights into transcriptional remodeling during ISR with broader implications for other stress responses. Through the IP-MS experiments, we determined the proteins that interacted with ATF4 and their change during ISR.
HostingRepository	iProX
AnnounceDate	2024-12-05
AnnouncementXML	Submission_2025-09-11_02:26:27.816.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peipei Jiang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-05 21:08:55	ID requested
⏵ 1	2025-09-11 02:26:28	announced

Dataset with its publication pending

submitter keyword: Integrated stress response, ATF4

Qian Bian
contact affiliation	Shanghai Jiao Tong University
contact email	qianbian@shsmu.edu.cn
lab head
Peipei Jiang
contact affiliation	Shanghai Jiao Tong University
contact email	17853139708@163.com
dataset submitter

iProX dataset URI