PXD058629-1

PXD058629 is an original dataset announced via ProteomeXchange.

Title	Pervasive divergence in protein thermostability is mediated by both structural changes and cellular environments
Description	Temperature is a universal environmental constraint and organisms have evolved diverse mechanisms of thermotolerance. A central feature of thermophiles relative to mesophiles is a universal shift in protein stability, implying that it is a major constituent of thermotolerance. However, organisms have also evolved extensive buffering systems, such as those that disaggregate and refold denatured proteins and enable survival of heat shock. Here, we show that both cellular and protein structural changes contribute to divergence in protein thermostability between two closely related Saccharomyces species that differ by 8°C in their thermotolerance. Using thermal proteomic profiling we find that 85% of S. cerevisiae proteins are more stable than their S. uvarum homologs and there is an average shift of 1.6°C in temperature induced protein aggregation. In an interspecific hybrid of the two species, S. cerevisiae proteins retain their thermostability, while the thermostability of their S. uvarum homologs is enhanced, indicating that cellular context contributes to protein stability differences. By purifying orthologous proteins we show that amino acid substitutions underlie melting temperature differences for two proteins, Guk1 and Aha1. Amino acid substitutions are also computationally predicted to contribute to stability differences for most of the proteome. Our results imply that coordinated changes in protein thermostability impose a significant constraint on the time scales over which thermotolerance can evolve.
HostingRepository	PRIDE
AnnounceDate	2025-01-10
AnnouncementXML	Submission_2025-01-10_08:42:24.162.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nilima Walunjkar
SpeciesList	scientific name: Saccharomyces uvarum; NCBI TaxID: 230603; scientific name: Saccharomyces cerevisiae x Saccharomyces uvarum; NCBI TaxID: 489137; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-05 19:57:54	ID requested
⏵ 1	2025-01-10 08:42:24	announced

Dataset with its publication pending

submitter keyword: S. uvarum,Thermal proteome profiling, yeast, S. cerevisiae, hybrid

Justin Fay
contact affiliation	Department of Biology, University of Rochester
contact email	jfay6@ur.rochester.edu
lab head
Nilima Walunjkar
contact affiliation	University of Rochetser
contact email	nwalunjk@ur.rochester.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058629
     1. Label: PRIDE project
     2. Name: Pervasive divergence in protein thermostability is mediated by both structural changes and cellular environments