PXD058623-1

PXD058623 is an original dataset announced via ProteomeXchange.

Title	Mechanosensitive interactions of tumoroids with an engineered environment promote cell proliferation and enhance drug response detection
Description	Despite remarkable advances in reproducing ever more sophisticated ex-vivo models, foundational challenges remain in standardizing and scaling up organoid culture. Here, we introduce a novel technology for high-throughput derivation and high-content analysis of tumoroids. This technology relied on microengineered tumoroid niches fabricated from PEG acrylate polymers by maskless photolithography to create a mechanically tunable microenvironment that is scalable and compatible with standard automated lab pipelines. We validated our innovative platform on patient-derived urothelial tumoroids and evaluated its use in high-end imaging approaches, including holotomography. Our results reveal that tumoroids dynamically interact and deform their synthetic environment, unravelling a mechanosensitive behavior of tumoroids depending on the features of their synthetic niche. Changing mechanical properties of microcavities modulated cell cycle dynamics and metabolism, as it affected their response to drugs, which highlights the critical role of mechanical stimuli in tumoroid development and future drug screening. Soft niches condition : M_N1/ M_N2/ M_N3/ M_N4 Stiff niches condition : S_N1/ S_N2/ S_N3/ S_N4 2D condition : 2D_N1/ 2D_N2/ 2D_N3/ 2D_N4
HostingRepository	PRIDE
AnnounceDate	2025-07-24
AnnouncementXML	Submission_2025-07-24_02:00:07.598.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume CHEVREUX
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methylthiolated residue; acetylated residue; monohydroxylated residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-05 10:15:37	ID requested
⏵ 1	2025-07-24 02:00:08	announced

10.1016/J.CELBIO.2025.100149;

submitter keyword: Mechanotransduction, Bladder cancer,ECM

Organoid, Urothelial cancer

Kristine Schauer
contact affiliation	Institut Gustave Roussy, Inserm U1279
contact email	Kristine.SCHAUER@gustaveroussy.fr
lab head
Guillaume CHEVREUX
contact affiliation	Institut Jacques Monod, CNRS UMR7592
contact email	guillaume.chevreux@ijm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058623
     1. Label: PRIDE project
     2. Name: Mechanosensitive interactions of tumoroids with an engineered environment promote cell proliferation and enhance drug response detection