PXD058622-1

PXD058622 is an original dataset announced via ProteomeXchange.

Title	Global maintenance of histone post-translational modifications during the transition into anoxia in embryos of the annual killifish Austrofundulus limnaeus
Description	Embryos of the annual killifish Austrofundulus limnaeus have the greatest tolerance to anoxia of all vertebrates, making them a powerful model to study the cellular mechanisms necessary for anoxia tolerance. However, the global histone landscape of this species has never been quantified or explored in relation to stress tolerance. Liquid chromatography-mass spectrometry, and a Python bioinformatics workflow, were used to identify histones and their post-translational modifications (hPTMs). This pipeline resulted in the detection of 267 unique biologically relevant histone post-translational modifications (unimod+residue). These PTMs represent sixteen types of biologically relevant hPTMs present during both anoxia and normoxia in Wourms’ Stage 36 embryos. This hPTM library presents an exciting opportunity to study histone modifications across development and in response to environmental stressors. No significant changes in PTM or histone abundance were observed between anoxic and normoxic embryos, suggesting that 24 hours of anoxia is not sufficient to induce epigenetic or histone isoform changes at the organismal level. This result is inconsistent with data presented for similar stresses in mammalian cells and thus stabilization of the hPTM landscape may be an adaptation that supports anoxia tolerance.
HostingRepository	PanoramaPublic
AnnounceDate	2026-01-23
AnnouncementXML	Submission_2026-01-23_19:21:19.572.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dietmar Kültz
SpeciesList	scientific name: Austrofundulus limnaeus; NCBI TaxID: 52670;
ModificationList	Dehydrated; Cation:Na; Carboxyethyl; Carbamidomethyl; Oxidation; Phospho; Dioxidation; ADP-Ribosyl; Acetyl; Deamidated; Gln->pyro-Glu; Delta:H(4)C(2); Carbamyl; GG; Dimethyl; Methyl; HNE; Trimethyl; Carboxymethyl; Biotin; Amidated
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-05 10:13:23	ID requested
⏵ 1	2026-01-23 19:21:20	announced

Hughes C, Mojica EA, K, ü, ltz D, Podrabsky JE, . Environ Epigenet, 11(1):dvaf015(2025) [pubmed]

submitter keyword: Mass spectrometry, epigenetics, histone post-translational modifications, anoxia, stress tolerance

Dietmar Kültz
contact affiliation	UC Davis
contact email	dkueltz@ucdavis.edu
lab head
Dietmar Kültz
contact affiliation	UC Davis
contact email	dkueltz@ucdavis.edu
dataset submitter

Panorama Public dataset URI