PXD058598-1

PXD058598 is an original dataset announced via ProteomeXchange.

Title	A conserved chronobiological complex times C. elegans development; map in vivo LIN-42 phosphorylation sites
Description	The mammalian PAS-domain protein PERIOD (PER) and its C. elegans orthologue LIN-42 have been proposed to constitute an evolutionary link between two distinct, circadian and developmental, timing systems. However, while the function of PER in animal circadian rhythms is well understood molecularly and mechanistically, this is not true for LIN-42’s function in timing rhythmic development. Here, using targeted deletions, we find that the LIN-42 PAS domains are dispensable for the protein’s function in timing molts. Instead, we observe arrhythmic molts upon deletion of a distinct sequence element, conserved with PER. We show that this element, designated CK1δ-binding domain (CK1BD), mediates stable binding to KIN-20, the C. elegans CK1δ/ε orthologue. We demonstrate that CK1δ phosphorylates LIN-42 and define two conserved helical motifs in the CK1BD, CK1BD-A and CK1BD-B, that have distinct roles in controlling CK1δ-binding and kinase activity in vitro. KIN-20 and the LIN-42 CK1BD are required for proper molting timing in vivo, and loss of LIN-42 binding changes KIN-20 subcellular localization. The interactions mirror the central role of a stable circadian PER–CK1 complex in setting a robust ~24-hour period. Hence, our results establish LIN-42/PER – KIN-20/CK1δ/ε as a functionally conserved signaling module of two distinct chronobiological systems.
HostingRepository	PRIDE
AnnounceDate	2025-10-10
AnnouncementXML	Submission_2025-10-10_02:35:59.312.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jan Seebacher
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: NEWT:6239;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-04 20:30:12	ID requested
⏵ 1	2025-10-10 02:35:59	announced

10.1038/S44318-025-00585-Z;

submitter keyword: KIN-20,LIN-42, CK1BD, phosphorylation, PAS-domain protein PERIOD, C. elegans development

Helge Grosshans
contact affiliation	Research Group Leader, Friedrich Miescher Institute for Biomedical Research
contact email	helge.grosshans@fmi.ch
lab head
Jan Seebacher
contact affiliation	FMI Basel
contact email	jan.seebacher@fmi.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058598
     1. Label: PRIDE project
     2. Name: A conserved chronobiological complex times C. elegans development; map in vivo LIN-42 phosphorylation sites