PXD058597-1

PXD058597 is an original dataset announced via ProteomeXchange.

Title	Human Flag-tagged RIG-I IP-MS in HEK293T
Description	nevaeh: The PARP inhibitor olaparib is widely used in cancer but is underused in treating lung adenocarcinoma (LUAD). Our initial analysis revealed that RIG-I, which is typically found in the cytoplasm and activates innate immunity, is linked to LUAD outcome. Here, we discovered that RIG-I undergoes lactylation, with the acetyltransferase PCAF serving as the lactylation writer. Treatment with the lactate transporter inhibitor syrosingopine prevents the efflux of lactic acid from cancer cells, increasing intracellular lactic acid, promoting RIG-I lactylation, and enhancing its interaction with the nuclear transport protein importin 8. The augmented interaction facilitates the nuclear translocation of RIG-I, diminishing PARP1 activity and enhancing the therapeutic effects when combined with olaparib. This synergy disrupts PARP1-mediated DNA repair mechanisms, increasing drug sensitivity and inhibiting tumor growth. The combination of olaparib and syrosingopine demonstrates better therapeutic efficacy than olaparib monotherapy in LUAD, providing a promising strategy for future LUAD treatments. nevaeh: HEK293T cells were transfected with Flag-RIG-I for 48 h, lysed in NP-40 buffer, and sonicated using a JY92-II sonicator. The supernatant was first incubated overnight with an anti-Flag conjugated with beads (Sigma, A2220) after being denatured. Following centrifugation at 3,000 rpm (4°C for 5 min), the supernatants were completely removed, and 60 μL of Flag–peptide was added to competitively bind to the beads. RIG-I protein was released, precipitated using Trichloroacetic acid (TCA), and subjected to mass spectrometry analysis for lactylation.
HostingRepository	PRIDE
AnnounceDate	2025-12-10
AnnouncementXML	Submission_2025-12-09_20:00:41.316.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058597
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	yulin li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-04 19:29:08	ID requested
⏵ 1	2025-12-09 20:00:41	announced

10.6019/PXD058597;

submitter keyword: HEK293T, IP-MS, RIG-I

Yulin Li
contact affiliation	1State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China 2Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing, China, Peking University, Beijing 100871, China
contact email	lylin@stu.pku.edu.cn
lab head
yulin li
contact affiliation	15653656728
contact email	lylin@stu.pku.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD058597

PRIDE project URI

• PRIDE
  1. PXD058597
     1. Label: PRIDE project
     2. Name: Human Flag-tagged RIG-I IP-MS in HEK293T