⮝ Full datasets listing
PXD058560-1
PXD058560 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Yeast phosphofructokinase subunit 2 has ATP-dependent RNA unwinding activity and modulates cell cycle progression |
| Description | Phosphofructokinase (PFK) is a rate-limiting glycolytic enzyme that also possesses an unexplored polyadenylated RNA binding activity. Here, we show that the two PFK subunits, Pfk1 and Pfk2, in budding yeast bind a large number of mRNAs in cells, including ones coding for proteins involved in regulation of mitotic cell cycle. Pkf2p directly binds to short GA-, UC-, AU- and U-rich motifs overrepresent in the mRNA targets in an Mg-ATP-dependent manner. Strikingly, Pfk2p displays directional 5 - 3 double-stranded RNA unwinding activity not seen with Pfk1p. Furthermore, Pfk2p dynamically associates with ribosomes and promotes translation of cell cycle genes. Consequently, pfk2, but not pfk1, mutant cells show severely delayed G1-S phase transition which is independent of the enzymes glycolytic activity. Our results uncovered a novel function of the yeast Pfk2 subunit, which acts as a translational activator of mitotic cell cycle genes possibly through energy-dependent RNA unwinding activity. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-02-19 |
| AnnouncementXML | Submission_2026-02-19_00:55:21.466.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Alex Schmidt |
| SpeciesList | scientific name: Saccharomycetales; common name: budding yeasts; NCBI TaxID: 4892; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-12-04 00:42:52 | ID requested | |
| ⏵ 1 | 2026-02-19 00:55:22 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: TMT, pfk2, DatasetType:Proteomics |
Contact List
| Alexander Schmidt | |
|---|---|
| contact affiliation | Biozentrum, Universtiy of Basel, 4056 Basel, Switzerland |
| contact email | alex.schmidt@unibas.ch |
| lab head | |
| Alex Schmidt | |
| contact affiliation | University of Basel |
| contact email | alex.schmidt@unibas.ch |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v07/MSV000096586/ |
