PXD058558 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The E3 ubiquitin ligase FBXL18 stabilizes BST2 to promote inflammation in RABV-infected astrocytes |
| Description | Lethal encephalitis caused by rabies virus (RABV) in mammals is known to be associated with the production of several pro-inflammatory cytokines, but the mechanism of such induction remains unclear. In this study, we found that RABV infects astrocytes which is the most abundant glial cell population and the dominant source of inflammatory factors in the central nervous system (CNS). A screen identified the E3 ubiquitin ligase FBXL18 as a critical factor responsible for RABV-induced inflammation. Mechanistically, infection by RABV up-regulates FBXL18, which induces K11-type ubiquitination of BST2 on Lys109 and Lys110, two residues that are also targeted by K33-type ubiquitination for degradation. FBXL18-mediated ubiquitination stabilizes BST2, leading to hyperphosphorylation of IκBα and excessive NF-κB activation. Knockdown of FBXL18 effectively inhibits IL-6 production and RABV replication. Our findings suggest that targeting FBXL18 is a potentially effective strategy for rabies treatment. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-29 |
| AnnouncementXML | Submission_2025-12-28_17:07:45.571.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Liu Yan |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-12-04 00:27:16 | ID requested | |
| ⏵ 1 | 2025-12-28 17:07:46 | announced | |
Publication List
| Wang J, Guo J, Li N, Tu Z, Liu S, Ma J, Zhang X, Cheng H, Hao Z, Feng Y, Lv S, Tai G, Zhou Y, Fu J, Tu C, Liu Y, The E3 ubiquitin ligase FBXL18 stabilizes BST2 to promote inflammation in RABV-infected astrocytes. Nat Commun, 16(1):11324(2025) [pubmed] |
| 10.1038/s41467-025-66427-y; |
Keyword List
| submitter keyword: FBXL18 |
| BST2 |
| NF-kB |
| Rabies virus |
| Ubiquitination |
Contact List
| Yan Liu |
|---|
| contact affiliation | Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, State Key Laboratory of Pathogen and Biosecurity, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, China. |
| contact email | liu820512@163.com |
| lab head | |
| Liu Yan |
|---|
| contact affiliation | liu820512@163.com |
| contact email | liu820512@163.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD058558 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058558
- Label: PRIDE project
- Name: The E3 ubiquitin ligase FBXL18 stabilizes BST2 to promote inflammation in RABV-infected astrocytes
