PXD058551-1

PXD058551 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomics of HepG2 Cells Reveals NGLY1 as an Important Regulator of Ferroptosis Resistance and Iron Uptake
Description	NGLY1 deficiency is a rare genetic disorder caused by mutations in the NGLY1 gene. This disorder presents a wide range of clinical symptoms, and its severity varies among affected individuals. Previous studies have focused on understanding the influence of NGLY1 on energy metabolism, revealing dysregulation in lipid metabolism following NGLY1 deletion. In this study, we investigated the consequences of the loss of NGLY1 on iron homeostasis using human hepatocellular carcinoma cells, HepG2. Comparative proteomics analysis revealed significant alterations in protein quantities in NGLY1-deficient HepG2 cells that suggested ferroptosis was prominently upregulated. Moreover, dysregulated iron uptake and increased reactive oxygen species (ROS) production were observed in the absence of NGLY1, indicating a novel perspective on the consequences of the loss of NGLY1. These findings provide important insights into the molecular pathways affected by NGLY1 deletion and may contribute to the development of potential therapeutic strategies for individuals with NGLY1 deficiency.
HostingRepository	PRIDE
AnnounceDate	2025-07-18
AnnouncementXML	Submission_2025-07-17_19:27:22.142.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058551
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Takehiro Suzuki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-03 20:40:03	ID requested
⏵ 1	2025-07-17 19:27:22	announced

10.6019/PXD058551;

submitter keyword: ferroptosis, HepG2 cells,NGLY1 deficiency, iron metabolism

Naoshi Dohmae
contact affiliation	Biomolecular characterization unit, Center for Sustainable Resource Science, RIKEN
contact email	dohmae@riken.jp
lab head
Takehiro Suzuki
contact affiliation	RIKEN Center for Sustainable Resource Science
contact email	takehirosuzuki@riken.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058551
     1. Label: PRIDE project
     2. Name: Comparative Proteomics of HepG2 Cells Reveals NGLY1 as an Important Regulator of Ferroptosis Resistance and Iron Uptake