PXD058535-1

PXD058535 is an original dataset announced via ProteomeXchange.

Title	Ubiquitination proteome profiling of Candida albicans
Description	Protein extraction The two groups (C, CS) were treated in same way, sample was first grinded with liquid nitrogen, then the powder was transferred to a 5-mL centrifuge tube and sonicated three times on ice using a high intensity ultrasonic processor (Scientz) in lysis buffer (including 1% TritonX-100, 10 mM dithiothreitol, and 1% protease inhibitor cocktail, 50 μM PR-619, 3 μM TSA, 50 mM NAM, 2 mM EDTA, and 1% phosphatase inhibitor for phosphorylation.). An equal volume of Tris-saturated phenol (pH 8.0) was added. Then, the mixture was further vortexed for 5 min. After centrifugation (4 °C, 10 min, 5 000g), the upper phenol phase was transferred to a new centrifuge tube. Proteins were precipitated by adding at least four volumes of ammonium sulfate-saturated methanol and incubated at -20 °C for at least 6 h. After centrifugation at 4 °C for 10 min, the supernatant was discarded. The remaining precipitate was washed with ice-cold methanol, followed by ice-cold acetone for three times. The protein was redissolved in 8 M urea and the protein concentration was determined with BCA kit according to the manufacturer’s instructions.
HostingRepository	PRIDE
AnnounceDate	2025-01-25
AnnouncementXML	Submission_2025-01-25_00:31:00.760.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yixin Zhang
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	ubiquitinylated lysine
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-03 10:06:22	ID requested
⏵ 1	2025-01-25 00:31:01	announced

Dataset with its publication pending

submitter keyword: Candida albicans, Streptococcus mutans, LC-MS/MS

Dr Xian Peng
contact affiliation	State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China (lab head)
contact email	2020224035084@stu.scu.edu.cn
lab head
Yixin Zhang
contact affiliation	15982217725
contact email	2020224035084@stu.scu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD058535

PRIDE project URI

• PRIDE
  1. PXD058535
     1. Label: PRIDE project
     2. Name: Ubiquitination proteome profiling of Candida albicans