PXD058518-1

PXD058518 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SH-SY5Y_CB1R-antagonist_Proteomics
Description	Cannabinoid receptor type 1 (CB1R) is one of the most abundant GPCRs in the brain, playing a critical role in neuronal viability. While CB1R activation is typically associated with neuroprotective effects, excessive or prolonged activation can be adverse. CB1R antagonists, therefore, have shown neuroprotective effects against neurotoxicity by CB1R overactivation. Unexpectedly, we found that CB1R antagonists rimonabant and AM251 induce cell death in human neuroblastoma SH-SY5Y cells under serum-free conditions, but not in serum-containing conditions. Phosphoproteomic analysis was performed to elucidate this molecular pathway. Phospho-EIF2AK3 (also known as PERK) was up-regulated in both rimonabant and AM251 treatments. PERK is a transducer of ER stress. Using western blotting it was also confirmed that the downstream effectors of PERK, eIF2α/ATF4/CHOP, were up-regulated, suggesting activation of the ER stress signal pathway PERK/eIF2α/ATF4/CHOP. In summary, cell death by CB1 antagonists under serum-free conditions results from ER stress response.
HostingRepository	jPOST
AnnounceDate	2024-12-03
AnnouncementXML	Submission_2024-12-03_02:45:07.743.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kazuaki Mori
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-03 02:45:03	ID requested
⏵ 1	2024-12-03 02:45:08	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Rimonabant, AM251, Hemopressin, Neuroblasoma cell, Proteomics

submitter keyword: Rimonabant, AM251, Hemopressin, Neuroblasoma cell, Proteomics

Contact List

Kosuke Kataoka
lab head
Kazuaki Mori
contact affiliation	Waseda University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003500/