PXD058468-1

PXD058468 is an original dataset announced via ProteomeXchange.

Title	Nigericin induced paraptosis-like cell death instead of pyroptosis in corneal keratocytes
Description	Corneal wound healing involves complex cellular processes, including keratocyte cell death, which plays a pivotal role in initiating tissue repair. Recent studies suggest that pyroptosis, characterized by the release of pro-inflammatory factors, occurs during tissue repair which may also contribute to the inflammatory phase of corneal wound healing. In this study, we investigated the nature of cell death in human keratocytes treated with nigericin, a known pyroptosis inducer. Surprisingly, nigericin triggered a unique form of non-inflammatory cell death marked by extensive vacuolation, resembling paraptosis. Proteomic analysis revealed that our human keratocytes lack key components of the canonical pyroptosis pathway, including NLRP3, ASC, and caspase-1, potentially explaining the absence of pyroptosis in these cells. This process was further confirmed by the absence of caspase-3 activation and lack of ER stress, alongside increased autophagic marker LC3-II accumulation. These findings suggest that nigericin triggers a paraptosis-like cell death in keratocytes, rather than pyroptosis, highlighting an alternative mechanism of cell death in corneal wound healing that warrants further exploration.
HostingRepository	PRIDE
AnnounceDate	2025-06-30
AnnouncementXML	Submission_2025-06-29_16:16:48.418.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaochun Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-02 02:23:55	ID requested
⏵ 1	2025-06-29 16:16:49	announced

Mi X, Zhou X, Zhu S, Mateus A, Backman LJ, Danielson P, Nigericin Induces Paraptosis-Like Cell Death Instead of Pyroptosis in Corneal Keratocytes. FASEB J, 39(12):e70740(2025) [pubmed]

10.1096/fj.202500502r;

submitter keyword: proteomics, corneal keratocytes,nigericin

Patrik Danielson
contact affiliation	Department of Medical and Translational Biology (MTB), Faculty of Medicine, Umeå University, 90187 Umeå, Sweden
contact email	patrik.danielson@umu.se
lab head
Shaochun Zhu
contact affiliation	Umea University
contact email	shaochunzhu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD058468

PRIDE project URI

• PRIDE
  1. PXD058468
     1. Label: PRIDE project
     2. Name: Nigericin induced paraptosis-like cell death instead of pyroptosis in corneal keratocytes