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PXD058450-1

PXD058450 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic Analysis of ACE2-Interacting Proteins During ER-to-PM Trafficking
Description	Using the RUSH (Retention Using Selective Hooks) method to study the secretion process of ACE2 from the endoplasmic reticulum to the plasma membrane, we transfected plasmids into cells and initiated secretion with biotin. ACE2 was released from the endoplasmic reticulum to the plasma membrane. We collected samples every 5 minutes within a 45-minute period, using cells without plasmid transfection as a control. We then employed Flag pull-down assays followed by mass spectrometry to identify the interacting proteins of ACE2 during this process.
HostingRepository	iProX
AnnounceDate	2024-11-29
AnnouncementXML	Submission_2024-12-01_23:09:27.577.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luming Wan
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-12-01 23:08:22	ID requested
⏵ 1	2024-12-01 23:09:27	announced

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: CoIP-MS, ACE2

Contact List

Congwen Wei
contact affiliation	Laboratory of Advanced Biotechnology, Beijing Institute of Biotechnology
contact email	weicongwen@aliyun.com
lab head
Luming Wan
contact affiliation	Laboratory of Advanced Biotechnology, Beijing Institute of Biotechnology
contact email	wanluming9@163.com
dataset submitter

Full Dataset Link List

iProX dataset URI