⮝ Full datasets listing
PXD058431-1
PXD058431 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | An integrated multi-proteomics approach reveals the altered N-glycosylation patterns during Jurkat T cell activation |
| Description | Protein glycosylation plays essential roles in regulating innate and adaptive immune response. Previous studies have typically focused on individual protein-glycan interaction or specific glycoform changes associated with T cell activation, yet a systematic characterization of protein glycosylation alterations remains insufficiently elucidated. To address these limitations, we conducted temporally-resolved quantitative multi-proteomic analysis on activated Jurkat T cells. Through an integrative analysis of glycoforms, site-specific glycans, glycoproteins, and glycosylation enzymes, we portrayed the dynamic landscape of protein glycosylation during Jurkat T cell activation. We found the heterogeneity and number of significantly up-regulated intact glycopeptides increased along with stimulation time. For most glycopeptides, their alteration patterns did not correlate well with the abundance of their glycoprotein substrates. Comparative analysis of global peptides, glycopeptides, and phosphopeptides revealed their distinctive changing patterns along Jurkat T cell activation, and only glycosylation demonstrated a steady increase trend with a large proportion of up-regulated glycopeptides. Collectively, this integrated multi-proteomics characterization of activated Jurkat T cells provided insights for the crucial role of glycosylation in modulating immune response, and would aid the development of novel therapeutic strategy targeting glycosylation. |
| HostingRepository | jPOST |
| AnnounceDate | 2025-11-30 |
| AnnouncementXML | Submission_2025-11-29_07:00:05.999.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | xiaoyan Liu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-11-29 21:29:49 | ID requested | |
| ⏵ 1 | 2025-11-29 07:00:06 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: multi-proteomics，N-glycosylation patterns，Jurkat T cell activation |
Contact List
| Mingliang Ye | |
|---|---|
| lab head | |
| xiaoyan Liu | |
| contact affiliation | Dalian Institute of Chemical Physics |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
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