PXD058417-1

PXD058417 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	S-palmitoylation of synaptic proteins in rat synaptoneurosomes
Description	Temporal and target-specific S-palmitoylation of synaptic proteins supports synapse and neural network plasticity: Synaptic plasticity is a phenomenon essential for learning and memory, which relies on activity-dependent changes in neural connectivity. S-palmitoylation, a reversible posttranslational lipid modification, regulates synaptic protein function by altering protein conformation, localization, trafficking, and their interactions. Despite its known significance in neuronal function, the temporal and protein-specific dynamics of S-palmitoylation during synaptic plasticity remain poorly understood. Proteomic analysis of synaptoneurosomes revealed a palmitoylome including over 700 proteins, with LTP-induced predominant depalmitoylation. Differentially palmitoylated proteins were associated with synaptic vesicle cycling, cytoskeletal dynamics, and neurotransmitter release. Synaptoneurosomes contained active palmitoylation machinery, supporting rapid, target-specific responses to NMDA receptor activation.
HostingRepository	PRIDE
AnnounceDate	2025-10-20
AnnouncementXML	Submission_2025-10-19_16:25:34.263.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	acetylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-29 09:01:10	ID requested
⏵ 1	2025-10-19 16:25:35	announced

Publication List

Pyty, ś A, Ijaz R, Buszka A, Mi, ł, ek J, Figiel I, Wardaszka-Pianka P, Roszkowska M, Mierzwa N, Wojtas A, Kerstein E, Serwa R, Kalita K, Dzakpasu R, Dziembowska M, W, ł, odarczyk J, W, ó, jtowicz T, Temporal and protein-specific S-palmitoylation supports synaptic and neural network plasticity. Cell Mol Life Sci, 82(1):355(2025) [pubmed]
10.1007/s00018-025-05893-5;

Pyty, ś A, Ijaz R, Buszka A, Mi, ł, ek J, Figiel I, Wardaszka-Pianka P, Roszkowska M, Mierzwa N, Wojtas A, Kerstein E, Serwa R, Kalita K, Dzakpasu R, Dziembowska M, W, ł, odarczyk J, W, ó, jtowicz T, Temporal and protein-specific S-palmitoylation supports synaptic and neural network plasticity. Cell Mol Life Sci, 82(1):355(2025) [pubmed]

10.1007/s00018-025-05893-5;

Keyword List

submitter keyword: LTP stimulation,Synaptoneurosomes, Acyl-biotin exchange, S-palmitoylation

submitter keyword: LTP stimulation,Synaptoneurosomes, Acyl-biotin exchange, S-palmitoylation

Contact List

Tomasz Wojtowicz
contact affiliation	Nencki Institute of Experimental Biology, Polish Academy of Sciences
contact email	t.wojtowicz@nencki.edu.pl
lab head
Remigiusz Serwa
contact affiliation	The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences
contact email	r.serwa@imol.edu.pl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD058417
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD058417

PRIDE project URI

Repository Record List

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