PXD058384-1

PXD058384 is an original dataset announced via ProteomeXchange.

Title	Identification of phosphatases that dephosphorylate Tom6 using synthetic trap-peptides
Description	The identification of phosphatases that dephosphorylate specific sites in proteins remains a major challenge, particularly for the major class of serine/threonine-specific phosphatases, which function as holoenzymes. Here, we report the development of synthetic trap-peptides to identify phosphatases that bind to Tom6, a subunit of the mitochondrial translocase of the outer membrane (TOM complex). The TOM complex is regulated by reversible phosphorylation, and while responsible kinases have been identified, the corresponding phosphatases so far remain unknown. Here, the trap-peptides enriched PP2A and PP4 as full holoenzymes from yeast cytosolic-fractions. We observed that their interaction with Tom6 was mediated through their regulatory subunits Psy2reg and Cdc55reg, and that PP2A was able to dephosphorylate Ser16 of Tom6 in vitro. In summary, synthetic trap-peptides facilitate the identification of complete holoenzymes that bind to the target sequence and reveal PP2A as the first TOM phosphatase.
HostingRepository	PRIDE
AnnounceDate	2025-06-16
AnnouncementXML	Submission_2025-06-16_01:03:34.595.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-28 08:44:16	ID requested
⏵ 1	2025-06-16 01:03:34	announced

Dataset with its publication pending

submitter keyword: trap-peptides,Phosphatases, Pfa, Psy2, PP2A, Cdc55, TOM complex, protein import, PP4, Tom6

Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS) TUM School of Life Sciences Technical University Munich (TUM) Gregor-Mendel-Straße 4 85354 Freising
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD058384

PRIDE project URI

• PRIDE
  1. PXD058384
     1. Label: PRIDE project
     2. Name: Identification of phosphatases that dephosphorylate Tom6 using synthetic trap-peptides