PXD058359-1

PXD058359 is an original dataset announced via ProteomeXchange.

Title	System-wide discovery of functional protein modifications by Refined-TPP
Description	Technique advances in proteomics including sample separation, mass spectrometry and searching algorithm have empowered in-depth discovery of protein post-translational modifications from biological samples. Instead, there is still a relative delay in the systematic researches for functional significance of these modifications. Herein, we propose Refined-TPP, a novel methodology for thermal proteomics, to systematically examine the effects of site-specific modification on the thermal stability of native proteins. This globally biophysical assay bridges the gap between the structural modifications and their functional impacts on proteins, and provides here a robust platform for the first time to effectively interrogate the functional implications for N-glycosylation. Finally, we elucidated the pivotal roles of explored N-glycosylation in protein structure and function, including protein stability, subcellular localization and enzyme activity. This novel strategy is readily applicable in a high-throughput and unbiased manner to further investigations for diverse objects, including drug-target screening, protein interactions and other functional PTMs exploration.
HostingRepository	iProX
AnnounceDate	2024-11-27
AnnouncementXML	Submission_2024-11-27_20:22:34.655.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guoli Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-27 20:22:05	ID requested
⏵ 1	2024-11-27 20:22:35	announced

Dataset with its publication pending

submitter keyword: thermal proteome profiling

Haojie Lu
contact affiliation	Institutes of Biomedical Sicences, Fudan University
contact email	luhaojie@fudan.edu.cn
lab head
Guoli Wang
contact affiliation	Institutes of Biomedical Sicences, Fudan University
contact email	19111510006@fudan.edu.cn
dataset submitter

iProX dataset URI