PXD058317-1

PXD058317 is an original dataset announced via ProteomeXchange.

Title	LiP-MS on CASK Purified from HEK293 cells treated with NR162
Description	Limited proteolysis coupled to mass spectrometry (LiP-MS) is a structural proteomics technique that can be used to identify the targets of small molecules. This is achieved by incubating lysate with the drug of interest, which induces structural changes such as occupation of the binding site or changes and protein-protein interactions. This is followed by a limited proteolysis step in which the unspecific protease proteinase K is added to the treated lysate. The protease preferentially cleaves accessible and flexible regions of the proteins, thus generating peptides that reflect the changes in surface accessibility caused by the treatment with the small molecule. Here we treated CASK Purified from HEK293 cells with the probe NR162, negative control NR187 and staurosporine to profile structural effects. This project is part of the IMI EUbOPEN project.
HostingRepository	PRIDE
AnnounceDate	2025-04-30
AnnouncementXML	Submission_2025-04-30_06:17:23.630.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Viviane Reber
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-27 02:33:12	ID requested
⏵ 1	2025-04-30 06:17:24	announced

Dataset with its publication pending

submitter keyword: chemical probe,LiP-MS

Matthias Gstaiger
contact affiliation	Institute of Molecular Systems Biology, Department of Biology, ETH Zurich, Zurich, Switzerland
contact email	matthias.gstaiger@imsb.biol.ethz.ch
lab head
Viviane Reber
contact affiliation	IMSB, ETH Zurich
contact email	reberv@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD058317

PRIDE project URI

• PRIDE
  1. PXD058317
     1. Label: PRIDE project
     2. Name: LiP-MS on CASK Purified from HEK293 cells treated with NR162