PXD058288-1

PXD058288 is an original dataset announced via ProteomeXchange.

Title	MS-based proteomic identification of NLRP3 binding partners in HeLa cells
Description	DNA double strand break (DSB) is a highly toxic lesion that can generate genome instability, a major source of tumorigenesis. DSBs are mainly repaired by nonhomologous end joining (NHEJ) or homologous recombination (HR). The selection of the DSB repair pathway primarily depends on the DNA resection of the DSB ends. Indeed, HR is initiated by resection at the DSB generating 3' single stranded extension. The shieldin complex prevents resection fostering DSB repair toward NHEJ. In the context of genome stability, NLRP3 was recently reported to interact with ATM and to enhance its activation in response to DNA DSBs. Whether NLRP3 controls the DSB repair machinery remains unknown. To further elucidate the function of NLRP3 in DNA repair, we sought NLRP3-binding partners by screening proteins that bind to FLAG-tagged NLRP3 (FLAG-NLRP3) in HeLa cells.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:08:47.030.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058288
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-26 06:51:13	ID requested
⏵ 1	2025-07-13 16:08:47	announced

Burlet D, Khan MM, Hacot S, Buthmann H, Bardoulet L, Huber AL, Gorry J, F, ö, hr B, Lopez BS, Cout, é Y, Faesen AC, Geyer M, Tissier A, Petrilli V, The inflammasome sensor NLRP3 interacts with REV7 to maintain genome integrity through homologous recombination. Nucleic Acids Res, 53(12):(2025) [pubmed]

10.6019/PXD058288;

10.1093/nar/gkaf554;

submitter keyword: co-immunoprecipitation,DNA double strand break, inflammasome, NLRP3, nanoLC-MS/MS

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD058288

PRIDE project URI

• PRIDE
  1. PXD058288
     1. Label: PRIDE project
     2. Name: MS-based proteomic identification of NLRP3 binding partners in HeLa cells