PXD058281-1

PXD058281 is an original dataset announced via ProteomeXchange.

Title	NANOS3-YTHDF2 mediated P-Body assembly disturbs folliculogenesis in offspring with maternal exposure to AAI
Description	Post-transcriptional RNA regulation is critical for the formation of primordial follicles, which determine female reproductive potential. Stress-induced disruptions in RNA metabolism are increasingly recognized as key factors impairing oogenesis, but the underlying mechanisms remain poorly understood. Here, we reveal that maternal exposure to aristolochic acid I (AAI), a reproductive toxicant commonly found in traditional medicines, disrupts P-body (PB) dynamics in neonatal mouse oocytes, resulting in impaired RNA metabolism during early folliculogenesis. These alterations contribute to defective primordial follicle formation and compromised oocyte competence in adulthood, ultimately compromising female fertility. Specifically, we identify NANOS3 and YTHDF2 as critical mediators of PB assembly, orchestrating RNA stabilization and degradation in response to AAI. Our findings demonstrate that YTHDF2, the m6A reader, facilitates the recruitment of NANOS3 to PBs, modulating the composition and functionality of these membraneless organelles. Proteomic analyses reveal that AAI induces aberrant associations of RNA-processing proteins with DDX6, a core PB component, altering the fate of transcripts with m6A modification during primordial follicle formation. This study highlights a mechanistic link between stress-induced PB assembly and RNA metabolism, providing new insights into how environmental factors influence female reproductive health. By uncovering the molecular basis of these processes, our work underscores the importance of RNA-centric regulatory networks in oogenesis and their vulnerability to external stressors.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:10:48.467.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JianWei Geng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-26 04:12:01	ID requested
⏵ 1	2026-09-06 16:10:49	announced

10.1186/s12964-026-02991-0;

Geng J, Zhang Y, He J, He F, Xiang Y, Wang Y, Geng Y, Ma Y, Dai Y, Chen X, Li F, Wang Y, Mu X, NANOS3-YTHDF2 drives aberrant P-body accumulation to impair folliculogenesis in offspring of maternal aristolochic acid I exposure. Cell Commun Signal, 24(1):(2026) [pubmed]

submitter keyword: Aristolochic acid Ⅰ

NANOS3

YTHDF2

P-body

Folliculogenesis

JianWei Geng
contact affiliation	ChongQing Medical University
contact email	1257156544@qq.com
lab head
JianWei Geng
contact affiliation	ChongQing Medical University
contact email	1257156544@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD058281

PRIDE project URI

• PRIDE
  1. PXD058281
     1. Label: PRIDE project
     2. Name: NANOS3-YTHDF2 mediated P-Body assembly disturbs folliculogenesis in offspring with maternal exposure to AAI