PXD058268-1

PXD058268 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Synergistic enhancement of anticancer effects by periodontal ligament stem cell exosomes and oxaliplatin via apoptosis induction and protein modulation
Description	Periodontal ligament stem cell-derived exosomes (PDLSC-exosomes) exhibit promising therapeutic potential in cancer treatment. Here, we characterize PDLSC-exosomes and investigate their effects on colorectal (HT-29, HCT-116) and oral (SCC-25) cancer cell lines. Nanoparticle tracking analysis and transmission electron microscopy revealed exosome diameters ranging from 80-220 nm and typical cup-shaped morphology. Flow cytometry confirmed surface markers CD9, CD63, and CD81. PKH67-labeling demonstrated efficient exosome internalization by all three cell lines. PDLSC-exosomes significantly inhibited cancer cell growth and colony formation in a dose-dependent manner. Synergistic effects were observed when combined with oxaliplatin, enhancing growth inhibition, apoptosis induction, and motility reduction. Apoptotic assays revealed increased Bax and p53 gene expression and altered protein profiles, particularly those involved in apoptosis and immune signaling pathways. Proteomic analysis identified differentially expressed proteins linked to translational processes and vesicle fusion. This study highlights the synergistic effects of PDLSC-exosomes and oxaliplatin, providing novel insights into their potential as an adjunct therapy to enhance cancer cell sensitivity and apoptosis.
HostingRepository	jPOST
AnnounceDate	2024-11-26
AnnouncementXML	Submission_2024-11-25_23:50:25.577.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dr.Wararat Chiangjong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-25 23:50:22	ID requested
⏵ 1	2024-11-25 23:50:25	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: PDLSC-exosomes, Oxaliplatin, Apoptosis, Colorectal cancer, Oral cancer, Proteomics

submitter keyword: PDLSC-exosomes, Oxaliplatin, Apoptosis, Colorectal cancer, Oral cancer, Proteomics

Contact List

Hathaitip Sritanaudomchai
lab head
Dr.Wararat Chiangjong
contact affiliation	Mahidol University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003485/