PXD058209-1

PXD058209 is an original dataset announced via ProteomeXchange.

Title	Paricalcitol and hydroxychloroquine modulates extracellular matrix and enhance chemotherapy efficacy in pancreatic cancer
Description	Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive cancer with poor prognosis and limited therapeutic options. In a previous publication, our group defined some of the mechanisms that vitamin D analogue paricalcitol (P) and hydroxychloroquine (H) potentiated the effects of gemcitabine-based chemotherapy in PDAC. Based on this, we hypothesized that the mechanisms of potentiation of 5-Fluorouracil (5FU) and oxaliplatin-based chemotherapy by PH may include different pathways than what was seen with gemcitabine-based therapy. Using MIA PaCa-2, HPAC and KPC cell lines, the combination of PH significantly increased the cell death, apoptosis, and S-phase cell cycle arrest. In vivo, the combination therapy inhibited PDAC growth and altered the immune landscape by activating T and NK cells. Proteomic analysis revealed that, significant reduction in ECM proteins, specifically integrin beta-4 (ITGB4) which was also seen with gemcitabine. Furthermore, genetic knockdown of ITGB4 suggested that the ECM inhibiti
HostingRepository	iProX
AnnounceDate	2024-11-23
AnnouncementXML	Submission_2024-11-24_21:22:03.749.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dhana Bandi
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-24 21:21:38	ID requested
⏵ 1	2024-11-24 21:22:04	announced

Dataset with its publication pending

submitter keyword: proteomics, paricalcitol, hydroxychloroquine, 5-FU, Oxaliplatin, pancreatic ductal adenocarcinoma

Bassel El-Rayes
contact affiliation	University of Alabama at Birmingham
contact email	BELRAYES@UAB.EDU
lab head
Dhana Bandi
contact affiliation	University of Alabama at Birmingham
contact email	dsbandi@uab.edu
dataset submitter

iProX dataset URI