PXD058198-1

PXD058198 is an original dataset announced via ProteomeXchange.

Title	Effect of Host Telomerase Inhibition on Human Cytomegalovirus
Description	Treatment options remain limited for Human Cytomegalovirus (HCMV). Host telomerase has been implicated in the pathogenesis and oncogenesis of multiple herpesviruses, most recently including HCMV. In this study, we investigated the effect of telomerase inhibition on HCMV replication, as well as the mechanism of the interaction between HCMV and host telomerase in vitro. We found that lytic HCMV infection increases host telomerase activity, at least in part through modulation of hTERT expression during earlier phases of the HCMV replication cycle. We found telomerase inhibition strongly reduced viral titer for two HCMV strains in a dose-specific manner. Both post-translational pharmaceutical telomerase inhibition and siRNA-mediated knockdown of hTERT reduce HCMV yield. Telomerase inhibition results in both reduction of viral gene and protein expression across the HCMV replication cycle, and suppressed viral genome replication and viral infectivity, suggesting interference with at least early steps of the HCMV viral life cycle. Altogether, our findings indicate telomerase plays an important, perhaps non-canonical role in lytic HCMV infection which includes the support of viral replication and infectivity.
HostingRepository	PanoramaPublic
AnnounceDate	2025-02-01
AnnouncementXML	Submission_2025-02-01_13:43:57.965.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Chloe Cavanaugh
SpeciesList	scientific name: Cytomegalovirus humanbeta5; NCBI TaxID: 3050295;
ModificationList	Carbamidomethyl
Instrument	Q Exactive HF; Dionex instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-24 15:52:15	ID requested
⏵ 1	2025-02-01 13:43:58	announced

no publication

submitter keyword: Cytomegalovirus, telomerase, telomerase inhibition.

Ileana Cristea
contact affiliation	Princeton University
contact email	icristea@princeton.edu
lab head
Chloe Cavanaugh
contact affiliation	Princeton University
contact email	chloec@princeton.edu
dataset submitter

Panorama Public dataset URI