PXD058195-1

PXD058195 is an original dataset announced via ProteomeXchange.

Title	The Actinobacillus pleuropneumoniae apxIV operon encodes an antibacterial toxin-immunity pair
Description	The ApxIVA protein belongs to a distinct class of a “clip and link” activity of Repeat-in-ToXin (RTX) exoproteins. Along with three other pore-forming RTX toxins (ApxI, ApxII and ApxIII), ApxIVA serves as a major virulence factor of Actinobacillus pleuropneumoniae, the causative agent of porcine pneumonia. The gene encoding ApxIVA is located on a bicistronic operon downstream of the orf1 gene and is expressed exclusively under in vivo conditions. Both ApxIVA and ORF1 are essential for full virulence, but the molecular mechanisms by which they contribute to the pathogenicity of A. pleuropneumoniae are not yet understood. Here, we provide a comprehensive structural and functional analysis of ApxIVA and ORF1 proteins. Our findings reveal that the N-terminal segment of ApxIVA shares a structural similarity with colicin M (ColM)-like bacteriocins and exhibits antimicrobial activity. The ORF1 protein resembles the colicin M immunity protein (Cmi) and, like Cmi, is exported to the periplasm through its N-terminal signal peptide. Additionally, ORF1 can protect bacterial cells from the antimicrobial activity of ApxIVA, suggesting that ORF1 and ApxIVA function as an antibacterial toxin-immunity pair. Moreover, we demonstrate that fetal calf serum can be utilized as a medium supplement to induce the production of ApxIVA and ORF1 proteins under in vitro conditions. These findings highlight the coordinated action of various RTX determinants, where the fine-tuned spatiotemporal production of ApxIVA may enhance the fitness of A. pleuropneumoniae, facilitating its invasion to a resident microbial community on the surface of airway mucosa.
HostingRepository	PRIDE
AnnounceDate	2024-12-28
AnnouncementXML	Submission_2024-12-28_09:36:22.182.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Jurnečka
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Actinobacillus pleuropneumoniae; NCBI TaxID: 715;
ModificationList	myristoylated residue; iodoacetamide derivatized residue
Instrument	Bruker Daltonics solarix series

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-24 02:32:12	ID requested
⏵ 1	2024-12-28 09:36:22	announced

10.1016/J.MICRES.2024.128043;

submitter keyword: A.pleuropneumoniae, RTX toxin

Laboratory of Molecular Biology of Bacterial Pathogens of the CAS, v.v.i.
contact affiliation	Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 00 Prague, Czech Republic.
contact email	bumba@biomed.cas.cz
lab head
David Jurnečka
contact affiliation	Institute of Microbiology, Czech Academy of Sciences
contact email	david.jurnecka@biomed.cas.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058195
     1. Label: PRIDE project
     2. Name: The Actinobacillus pleuropneumoniae apxIV operon encodes an antibacterial toxin-immunity pair