PXD058194 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The adaptor protein AP-3β disassembles heat-induced stress granules via 19S RP-associated deubiquitylation in Arabidopsis |
Description | To survive under adverse conditions, plants form stress granules (SGs) to temporally store mRNA and halt translation as a primary response. Dysregulation in SG disassembly can have detrimental effects on plant survival after stress release, yet the underlying mechanism remains poorly understood. Using Arabidopsis as a model system, we demonstrated that the AP-3 subunit AP-3β participates in heat response independently of its conventional role in vacuolar transportinteracts with the SG core RNA-binding proteins TSN1/2 in vivo and in vitro. We also showed that AP-3β is rapidly recruited to SGs upon heat induction and plays a key role in SG disassembly after heat release. We also discovered thatGenetic evidences support that AP-3β serves as an adaptor to recruit the 19S regulatory particle (RP) of the proteasome to SGs upon heat induction. Notably, the 19S RP promotes SG disassembly through RP-associated deubiquitylation, independent of its proteolytic catalytic activity. This deubiquitylation process of SG components is crucial for translation reinitiation and growth recovery after heat release. Our findings shed light on the non-proteolytic catalytic function of the RP in regulating SG dynamics disassembly and reveal highlight the importance of endomembrane proteins in supporting RNA granule dynamics in plant cells.a non-degradation mechanism for cellular adaptation to environmental stresses. |
HostingRepository | PRIDE |
AnnounceDate | 2025-01-24 |
AnnouncementXML | Submission_2025-01-23_22:05:37.552.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lei Pang |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | ubiquitinylated lysine |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-11-23 23:22:44 | ID requested | |
⏵ 1 | 2025-01-23 22:05:37 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: stress granules |
AP-3β |
TSN1/2 |
19S RP |
deubiquitylation |
Contact List
Ruixi Li |
contact affiliation | Shenzhen Key Laboratory of Plant Genetic Engineering and Molecular Design, Institute of Plant and Food Science, Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China |
contact email | lirx@sustech.edu.cn |
lab head | |
Lei Pang |
contact affiliation | Shenzhen Key Laboratory of Plant Genetic Engineering and Molecular Design, Institute of Plant and Food Science, Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, China |
contact email | pangl@sustech.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD058194
- Label: PRIDE project
- Name: The adaptor protein AP-3β disassembles heat-induced stress granules via 19S RP-associated deubiquitylation in Arabidopsis