PXD058193-1

PXD058193 is an original dataset announced via ProteomeXchange.

Title	Use of the proteomics approach to study endometrial cancer.
Description	Endometrial cancer (EC) is the second most frequent gynecological malignant tumor in postmenopausal women. Pathogenic mechanisms related to the onset and development of disease are still unknown. In this study, we aimed to characterize the EV proteome by combining Data-Independent Acquisition (DIA) acquisition, in albumin-depleted serum EVs to identify dysregulated proteins and enzymes associated with the disease. A deep proteomics analysis with advanced computational tools allowed us to identify a large number of proteins in serum albumin-depleted extracellular vesicles (EVs) from 10 patients with EC compared to 10 healthy controls. This is the largest number of proteins identified in EC serum EVs. After quantification and statistical analysis, we identified 373 significantly (p < 0.05) dysregulated proteins involved in neutrophil and platelet degranulation pathways. A more detailed bioinformatics analysis revealed 61 dysregulated enzymes related to metabolic and catabolic pathways linked to tumor invasion. Our bioinformatic analysis identified 49 metabolic and catabolic pathways related to tumor growth. MTD project_tag Cancer (B/D-HPP)
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_05:00:51.931.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michelangelo Aloisio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-23 19:39:44	ID requested
⏵ 1	2025-05-07 05:00:53	announced

10.3390/biomedicines13010095;

Kharrat F, Capaci V, Conti A, Golino V, Campiglia P, Balasan N, Aloisio M, Licastro D, Monasta L, Caponneto F, Beltrami AP, Romano F, di Lorenzo G, Ricci G, Ura B, A Pilot Study of Exosome Proteomic Profiling Reveals Dysregulated Metabolic Pathways in Endometrial Cancer. Biomedicines, 13(1):(2025) [pubmed]

ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: proteomics

mass spectrometry

extracellular vesicles

Blendi Ura
contact affiliation	Institute for Maternal and Child Health IRCCS “Burlo Garofolo” Department of gynecology 34137 Trieste, Italy
contact email	blendi.ura@burlo.trieste.it
lab head
Michelangelo Aloisio
contact affiliation	National Institute of Gastroenterology S. De Bellis, IRCCS Research Hospital,
contact email	michelangelo.aloisio@irccsdebellis.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD058193

PRIDE project URI

• PRIDE
  1. PXD058193
     1. Label: PRIDE project
     2. Name: Use of the proteomics approach to study endometrial cancer.