PXD058189-1

PXD058189 is an original dataset announced via ProteomeXchange.

Title	Plant defense against nematodes: Crucial role of Heat Shock Proteins and adaptive strategies
Description	Guava (Psidium guajava), referred to as the "tropical apple," is esteemed for its sweet flavor, nutritional density, and medicinal attributes, being rich in ascorbic acid, phenolics, carotenoids, fibers, and minerals. Despite its agricultural significance, guava cultivation faces considerable challenges from plant-parasitic nematodes (PPN), particularly root-knot nematodes (RKN; Meloidogyne spp.). In South America, Meloidogyne enterolobii causes severe root damage and economic losses to this crop. Plants fight nematodes through complex immune mechanisms involving pattern recognition receptors (PRRs) and signaling pathways, such as pattern-triggered immunity (PTI). The present research employed comparative shotgun proteomic analysis complemented by microscopic imaging and histochemical assays of roots from susceptible Psidium guajava and resistant P. guineense, inoculated or not with M. enterolobii, Plant-nematode interactions revealed intricate cellular responses such as giant cells formation, hypersensitivity reactions, and biochemical pathway adjustments in sucrose transport and antioxidant enzyme activities. Synthesis and accumulation of secondary metabolites like terpenes, alkaloids, and phenolics in inoculated and resistant plants were positively correlated to plant resilience. The 70 kDa heat shock protein (HSP70) emerged as pivotal in plant response, being upregulated during nematode infection.
HostingRepository	PRIDE
AnnounceDate	2025-09-08
AnnouncementXML	Submission_2025-09-07_20:04:36.333.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kátia Valevski Sales Fernandes
SpeciesList	scientific name: Psidium guajava; NCBI TaxID: 120290; scientific name: Psidium cattleyanum; NCBI TaxID: 375274;
ModificationList	No PTMs are included in the dataset
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-22 19:04:17	ID requested
⏵ 1	2025-09-07 20:04:38	announced

de Oliveira Costa SN, da Paschoa RP, Alexandrino CR, Cremonez PM, Ribeiro JM, da Cunha E Castro JM, Da Cunha M, Silveira V, Oliveira AEA, Fernandes KVS, Psidium Defenses Against Meloidogyne enterolobii: Proteomic and Microscopic Analysis of this Plant-Predator Association. Proteomics, 25(16):17-27(2025) [pubmed]

10.1002/pmic.70015;

submitter keyword: Guava (Psidium guajava). Plant-parasitic nematodes. Immune mechanisms. Root anatomy. Proteomic analysis. Crop resistance.

Kátia Valevski Sales Fernandes
contact affiliation	Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego, Campos dos Goytacazes, RJ, Brazil.
contact email	cowpkat@uenf.br
lab head
Kátia Valevski Sales Fernandes
contact affiliation	Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego, Campos dos Goytacazes, RJ, Brazil.
contact email	cowpkat@uenf.br
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD058189

PRIDE project URI

• PRIDE
  1. PXD058189
     1. Label: PRIDE project
     2. Name: Plant defense against nematodes: Crucial role of Heat Shock Proteins and adaptive strategies