PXD058171-1

PXD058171 is an original dataset announced via ProteomeXchange.

Title	Salivary proteomic profile related to excessive gingival bleeding in pregnant women with obesity: insights into biological mechanisms assessed by Tandem Mass Spectrometry
Description	Objective: To investigate the salivary proteomic profile associated with excessive gingival bleeding in pregnant women with obesity. Methods: Participants were divided into two groups based on Bleeding on Probing (BOP): excessive gingival bleeding (BOP > 50%, G1 = 9) and no excessive gingival bleeding (BOP 0–30%, G2 = 9). Unstimulated saliva samples were collected and individually analyzed using Nano Liquid Chromatography Electron Spray Ionization Tandem Mass Spectrometry (nLC-ESI-MS/MS). Proteins were classified by Gene Ontology (GO) analysis for biological process, molecular function, immune system involvement, and cellular component. Differences in protein expression were identified using thresholds of p > 0.05 for downregulation and 1-p < 0.95 for upregulation. Results: Among 183 identified proteins, 100 were shared between groups, with 57 up-regulated and 27 down-regulated in G1. Key biological processes included the antimicrobial humoral response and hydrogen peroxide catabolism, with proteins linked to immune function and endopeptidase regulation. Interaction network analysis revealed Lactotransferrin (increased 5-fold in G1), Haptoglobin (4-fold), and Immunoglobulin J chain (3-fold) as up-regulated, while Statherin (5-fold) and Protein S100-A8 (4-fold) were down-regulated. These proteins were associated with defense response and antimicrobial activity. Conclusions: Pregnant women with obesity and excessive gingival bleeding exhibited distinct salivary proteomic profiles, characterized by upregulation of immune-related proteins and downregulation of tissue-protective proteins. These findings highlight potential biomarkers for early detection and targeted management of periodontal inflammation in this high-risk group.
HostingRepository	PRIDE
AnnounceDate	2025-06-23
AnnouncementXML	Submission_2025-06-22_16:13:58.620.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerson Foratori Junior
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Xevo G2 Q-Tof; nanoACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-22 04:19:51	ID requested
⏵ 1	2025-06-22 16:13:59	announced

10.1590/1678-7757-2025-0031;

Marchi LT, Pirondi AB, Ventura TMO, Grizzo LT, Buzalaf MAR, Foratori-Junior GA, Generalized gingivitis-related salivary proteomic profile in pregnant women with obesity: insights into biological mechanisms assessed by Tandem Mass Spectrometry. J Appl Oral Sci, 33():e20250031(2025) [pubmed]

submitter keyword: Saliva, Pregnancy, Obesity, Proteomics,Gingivitis

Gerson Aparecido Foratori Junior
contact affiliation	Bauru School of Dentistry from University of São Paulo
contact email	gerson.foratori@usp.br
lab head
Gerson Foratori Junior
contact affiliation	Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo
contact email	gerson.foratori@usp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058171
     1. Label: PRIDE project
     2. Name: Salivary proteomic profile related to excessive gingival bleeding in pregnant women with obesity: insights into biological mechanisms assessed by Tandem Mass Spectrometry