PXD058169-1

PXD058169 is an original dataset announced via ProteomeXchange.

Title	Neuronal processes contain the essential components for the late steps of ribosome biogenesis
Description	Neurons rely on spatial and temporal control of protein synthesis to respond rapidly and locally to external stimuli, a process facilitated by the dynamic localization and modification of ribosomes. While previous research has shown that neuronal activity can regulate ribosome localization and modify translation rates, little is known about ribosomal assembly within neuronal processes. Here, we investigated the potential for local ribosome maturation in rat neurons using proteomics, RNA sequencing, and imaging techniques. We detected an abundance of ribosome biogenesis factors (RBFs) in distal neuronal compartments, particularly those associated with the late stages of ribosome assembly. Moreover, we detected cytosolic pre-rRNA species in dendrites, alongside enzymes necessary for their processing, suggesting that local ribosome maturation can occur far from the nucleus. These findings challenge conventional models that confine ribosome biogenesis to nuclear and perinuclear regions and suggest that neurons may fine-tune local protein synthesis by regulating ribosome assembly near synaptic sites. This mechanism may enable rapid modulation of the translational capacity in response to physiological changes, regulating synaptic plasticity and local protein synthesis in neurons.
HostingRepository	PRIDE
AnnounceDate	2025-08-11
AnnouncementXML	Submission_2025-08-11_02:18:23.126.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-22 03:45:30	ID requested
⏵ 1	2025-08-11 02:18:23	announced

10.1073/pnas.2502424122;

Fusco CM, Staab A, Bourke AM, Tushev G, Desch K, Lins EM, Ciirdaeva E, Tom Dieck S, Kaltenschnee N, Heckel A, Langer JD, Schuman EM, Neuronal processes contain the essential components for the late steps of ribosome biogenesis. Proc Natl Acad Sci U S A, 122(31):e2502424122(2025) [pubmed]

submitter keyword: neuron, ribosome enrichment,proteomics, soma, ribosome biogenesis, data-independent acquisition, dendrites

Julian Langer
contact affiliation	MPI for Brain Research, Proteomics, Frankfurt, Germany
contact email	julian.langer@brain.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD058169

PRIDE project URI

• PRIDE
  1. PXD058169
     1. Label: PRIDE project
     2. Name: Neuronal processes contain the essential components for the late steps of ribosome biogenesis