PXD058147-1

PXD058147 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of ConcanamycinA-treated macrophages that have phagocytosed SILAC-bacteria
Description	Macrophage are phagocytic cells that reprogram their cellular metabolism depending on the nature of innate immune receptors engaged upon microbial encounter. Yet, whether engulfed microbes constitute a source of nutrients for macrophages and how this contribute at establishing a specific metabolic reprogramming is poorly defined. Our study aims at understanding how microbial-derived amino acids feed macrophage metabolism by tracing labelled microbial amino acid in macrophage proteins. Here we compared the incorporation of labelled amino acids derived from dead bacteria into macrophage treated or not with the v-ATPase ConcanamacinA.
HostingRepository	PRIDE
AnnounceDate	2025-04-18
AnnouncementXML	Submission_2025-04-18_01:41:25.362.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-21 10:46:34	ID requested
⏵ 1	2025-04-18 01:41:26	announced

Lesbats J, Brillac A, Reisz JA, Mukherjee P, Lhuissier C, Fern, á, ndez-Monreal M, Dupuy JW, Sequeira A, Tioli G, De La Calle Arregui C, Pinson B, Wendisch D, Rousseau B, Efeyan A, Sander LE, D'Alessandro A, Garaude J, Macrophages recycle phagocytosed bacteria to fuel immunometabolic responses. Nature, 640(8058):524-533(2025) [pubmed]

10.1038/s41586-025-08629-4;

submitter keyword: innate immunity, phagocytosis,macrophages, SILAC

Johan Garaude
contact affiliation	University of Bordeaux, INSERM, MRGM, U1211, F-33000 Bordeaux, France. ImmunoConcEpT, CNRS UMR 5164, INSERM ERL 1303, University of Bordeaux, Bordeaux, France.
contact email	johan.garaude@inserm.fr
lab head
Dupuy Jean-William
contact affiliation	OncoProt plateform, UAR TBMCore CNRS 3427 INSERM US005 University of Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD058147

PRIDE project URI

• PRIDE
  1. PXD058147
     1. Label: PRIDE project
     2. Name: Proteomic analysis of ConcanamycinA-treated macrophages that have phagocytosed SILAC-bacteria