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PXD058127-1

PXD058127 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePolystyrene Microplastics effects on human gingival fibroblasts
DescriptionHuman gingival fibroblast cells were treated with 50 micro grams per mL of 1 micro meter polystyrene microplastics, and the control (with only the medium) after 48, and 72 hours the cells were collected to obtain a pellet. Total cell extracts were obtained by treating cells with RIPA buffer 0.1 SDS plus protease and phosphatase inhibitors then incubated on ice. After centrifuging at12000 rpm for 20min at 4C. The Pierce BCA Protein Assay Kit to determine the protein concentration. A volume corresponding to 25 micro grams of proteins was loaded into a Nanosep 10-kDa-cutoff filter and digested according to our routine protocol. Each digested protein sample was analyzed in technical triplicate. Raw data were processed using PEAKS studio using the correct precursor only option. Spectra were matched against the UniProt SwissProt database restricted to Homo sapiens taxonomy, to which a list of common contaminants was appended.
HostingRepositoryMassIVE
AnnounceDate2026-04-20
AnnouncementXMLSubmission_2026-04-20_00:40:21.613.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMaurizio Ronci
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Oxidation
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-11-21 03:25:31ID requested
12026-04-20 00:40:22announced
Publication List
no publication
Keyword List
submitter keyword: human gingival fibroblast, microplastics, polystyrene, DatasetType:Proteomics
Contact List
maurizio ronci
contact affiliationuniversity g.d'annunzio of Chieti-Pescara Italy
contact emailmaurizio.ronci@unich.it
lab head
Maurizio Ronci
contact affiliationUniversity G. d'Annunzio ITALY
contact emailmaurizio.ronci@unich.it
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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