PXD058097-1

PXD058097 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Hydrogen-deuterium exchange mass spectrometry of rotavirus NSP2/NSP5 biomolecular condensates
Description	Biomolecular condensates selectively compartmentalise and organise biomolecules within the crowded cellular milieu, and are instrumental in some disease mechanisms, including aiding RNA virus replication. Upon infection, many RNA viruses form biomolecular condensates that are often referred to as viral factories. The assembly mechanism of these viral factories remains poorly defined, but involves transient, non-stoichiometric protein/RNA interactions, posing challenges for their characterisation. Here we present HDX-MS data of NSP2 and NSP5, in a biomolecular condensate to study the mechanism of condensate assembly.
HostingRepository	PRIDE
AnnounceDate	2025-06-23
AnnouncementXML	Submission_2025-06-22_16:25:49.322.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antonio Calabrese
SpeciesList	scientific name: Bovine rotavirus strain RF; NCBI TaxID: 10933;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-20 09:21:42	ID requested
⏵ 1	2025-06-22 16:25:50	announced

Publication List

10.1002/pro.70181;
Colyer A, Acker J, Borodavka A, Calabrese AN, Uncovering protein conformational dynamics within two-component viral biomolecular condensates. Protein Sci, 34(7):e70181(2025) [pubmed]

10.1002/pro.70181;

Colyer A, Acker J, Borodavka A, Calabrese AN, Uncovering protein conformational dynamics within two-component viral biomolecular condensates. Protein Sci, 34(7):e70181(2025) [pubmed]

Keyword List

submitter keyword: rotavirus, NSP2,HDX, NSP5

submitter keyword: rotavirus, NSP2,HDX, NSP5

Contact List

Antonio Calabrese
contact affiliation	Astbury Centre for Structural Molecular Biology, School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK
contact email	a.calabrese@leeds.ac.uk
lab head
Antonio Calabrese
contact affiliation	School of Molecular and Cellular Biology, University of Leeds
contact email	a.calabrese@leeds.ac.uk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD058097

PRIDE project URI

Repository Record List

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