PXD058095-1

PXD058095 is an original dataset announced via ProteomeXchange.

Title	Identification of immunogenic markers in Toxoplasma gondii bradyzoite proteins
Description	Toxoplasma gondii, a protozoan parasite, causes toxoplasmosis, a widespread zoonotic and food-borne disease that poses significant risks, particularly in opportunistic and congenital cases. This obligate intracellular parasite is highly prevalent worldwide, largely due to its versatile life cycle, involving multiple hosts and transmission routes, and its ability to establish chronic infections. The presence of this neurotropic parasite in the brain poses a reactivation risk in immunocompromised individuals and is associated with a higher likelihood of developing mental disorders. However, the role of the dormant bradyzoite stage in the pathophysiology of the disease is underexplored, mainly due to the lack of non-invasive detection methods and serologic tests targeting bradyzoite- or cyst-specific antigens. In tachyzoites, MORC partners with histone deacetylase HDAC3 to restrict chromatin access to transcription machinery at genes specific to the sexual and chronic stages. Depletion of MORC results in significant transcriptional changes, leading to the expression of a large repertoire of bradyzoite-specific genes, encompassing up to 50% of the chronic sub-transcriptome. We hypothesized that reducing MORC levels could be an effective strategy to discover new immunogenic bradyzoite markers. To test this, we identified bradyzoite proteins inducing immunoreactivity of sera from chronically infected, BCLA-positive mice.
HostingRepository	PRIDE
AnnounceDate	2025-08-01
AnnouncementXML	Submission_2025-08-01_05:26:20.971.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058095
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-20 08:48:35	ID requested
⏵ 1	2025-08-01 05:26:21	announced

10.6019/PXD058095;

10.1038/s44321-025-00252-0;

Robert MG, Swale C, Pachano B, D, é, p, é, ry L, Bellini V, Dard C, Cannella D, Corrao C, Belmudes L, Cout, é Y, Bougdour A, Pelloux H, Chapey E, Wallon M, Brenier-Pinchart MP, Hakimi MA, Uncovering biomarkers for chronic toxoplasmosis detection highlights alternative pathways shaping parasite dormancy. EMBO Mol Med, 17(7):1686-1715(2025) [pubmed]

submitter keyword: serological marker,Toxoplasma gondii, Toxoplasmosis, bradyzoite, nanoLC-MS/MS

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058095
     1. Label: PRIDE project
     2. Name: Identification of immunogenic markers in Toxoplasma gondii bradyzoite proteins