PXD058040-1

PXD058040 is an original dataset announced via ProteomeXchange.

Title	Map of the Neuronal Tissue O-glycoproteome Reveal Specific Traits in the Secretogranin Family
Description	Protein O-glycosylation is an abundant PTM of the central nervous system and impairments in O-glycosylation cause severe developmental disorders most with prominent neurological involvement. Despite recent advancements, lack of insight into targets of protein O-glycosylation in neuronal tissues severely hampers progression in unravelling disease etiology. Here we report a comprehensive map of GalNAc-type O-glycoproteins (>800) and O-glycosites (>4,000) from neuronal tissues and cell lines compiled in a web-based resource. We identified abundant O-glycosites within major classes of proteins involved in neuroplasticity such as perineural nets, synapse formation, axon guidance, membrane remodeling and dense core granulogenesis. We demonstrate that Chromogranin A, a key player in neurotransmitter signaling, has abundant O-GalNAc as well as glycosaminoglycan glycosylation, and that these modifications are important for proper multimerization. In agreement with this, neuronal cell lines deficient in O-glycosylation exhibit higher capacity for storing the neurotransmitter noradrenaline and enlarged neurotransmitter-containing dense core granules.
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-27_16:05:34.196.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonas Nilsson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-19 04:58:11	ID requested
⏵ 1	2025-07-27 16:05:35	announced

10.1016/j.jbc.2025.110313;

Madsen TD, Topaktas AB, Dworkin LA, Hintze J, Hansen LH, Nikpour M, Lackman JJ, Goth CK, Larson G, Shiplett EJ, Edmondson AC, Zhou Z, Miller RL, Joshi HJ, Vakhrushev SY, Schjoldager KT, Map of the neuronal O-glycoproteome reveals driver functions in the regulated secretory pathway. J Biol Chem, 301(7):110313(2025) [pubmed]

submitter keyword: neurotransmitter,Granin, central nervous system, glycosaminoglycans, O-glycosylation, CDG, neuroplasticity, dense core granules, domaine, PNN

Katrine Schjoldager
contact affiliation	Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark.
contact email	schjoldager@sund.ku.dk
lab head
Jonas Nilsson
contact affiliation	Proteomics Core Facility, University of Gothenburg
contact email	jonas.gm.nilsson@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD058040

PRIDE project URI

• PRIDE
  1. PXD058040
     1. Label: PRIDE project
     2. Name: Map of the Neuronal Tissue O-glycoproteome Reveal Specific Traits in the Secretogranin Family