PXD058012-1

PXD058012 is an original dataset announced via ProteomeXchange.

Title	Inhibition of Cardiac p38 Highlights the Role of the Phosphoproteome in Heart Failure Progression
Description	We employed a guinea pig model of heart failure (HF) that combines ascending aortic constriction with daily administration of the β-adrenergic agonist isoproterenol (1 mg/kg/day for 1 hour) via an implanted programmable pump. The experimental groups included: 1) Sham-operated controls; 2) ACi (Aortic Constriction + isoproterenol); and 3) ACi-SB (ACi with SB treatment delivered via an implanted osmotic pump at 0.5 mg/kg/day). To investigate the effects of P38 MAPK inhibition on the HF proteome and phosphoproteome, we performed a comprehensive 16-plex Tandem Mass Tag (TMT) analysis across these three groups. Phosphopeptide enrichment was carried out on 80% of the pooled sample using titanium dioxide (TiO2), while the remaining unenriched 20% was utilized for protein expression analysis. A 2D-LC-MS/MS workflow was employed to achieve detailed proteomic and phosphoproteomic profiling.
HostingRepository	PRIDE
AnnounceDate	2025-09-08
AnnouncementXML	Submission_2025-09-07_18:00:27.068.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD058012
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sogol Sedighi
SpeciesList	scientific name: Cavia porcellus (Guinea pig); NCBI TaxID: 10141;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-18 14:51:31	ID requested
⏵ 1	2025-09-07 18:00:27	announced

10.1021/acsomega.5c03687;

Sedighi S, Liu T, O'Meally R, Cole RN, O'Rourke B, Foster DB, Inhibition of Cardiac p38 Highlights the Role of the Phosphoproteome in Heart Failure Progression. ACS Omega, 10(32):36082-36097(2025) [pubmed]

10.6019/PXD058012;

ProteomeXchange project tag: Cardiovascular (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Heart Failure,Guinea Pig, Heart , P38 Inhibition, TMT , LC-MS/MS

D. Brian
contact affiliation	Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, MD 20205, USA
contact email	dbrianfoster@jhmi.edu
lab head
Sogol Sedighi
contact affiliation	Division of Cardiology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
contact email	ssedigh2@jh.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD058012
     1. Label: PRIDE project
     2. Name: Inhibition of Cardiac p38 Highlights the Role of the Phosphoproteome in Heart Failure Progression