PXD058003-1

PXD058003 is an original dataset announced via ProteomeXchange.

Title	Comparative single-cell and spatial transcriptomics illuminate bat immunity and barrier tissue evolution
Description	Bats have adapted to pathogens through diverse mechanisms, including increased resistance - rapid pathogen elimination, and tolerance - limiting tissue damage following infection. In the Egyptian fruit bat (an important model in comparative immunology) several mechanisms conferring disease tolerance were discovered, but mechanisms underpinning resistance remain poorly understood. Previous studies on other species suggested that elevated basal expression of innate immune genes may lead to increased resistance to infection. Here, we test whether such transcriptional patterns occur in Egyptian fruit bat tissues through single-cell and spatial transcriptomics of gut, lung and blood cells, comparing gene expression between bat, mouse and human. Despite numerous recent loss and expansion events of interferons in the bat genome, interferon expression and induction are remarkably similar to that of mouse. In contrast, central complement system genes are highly and uniquely expressed in key regions in bat lung and gut epithelium, unlike in human and mouse. These genes also evolve rapidly in their coding sequence across the bat lineage. Finally, the bat complement system displays strong hemolytic and inhibitory activities. Together, these results indicate a distinctive transcriptional divergence of the complement system, which may be linked to bat resistance, and highlight the intricate evolutionary landscape of bat immunity.
HostingRepository	PRIDE
AnnounceDate	2024-11-18
AnnouncementXML	Submission_2024-11-18_15:22:30.257.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Moon
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat); NCBI TaxID: 9407;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series; timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-18 11:45:39	ID requested
⏵ 1	2024-11-18 15:22:30	announced

Dataset with its publication pending

submitter keyword: proteomics,bat, immunity, LC-MS/MS

Leonard J. Foster
contact affiliation	Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Canada
contact email	foster@msl.ubc.ca
lab head
Jenny Moon
contact affiliation	University of British Columbia
contact email	kyungmee@mail.ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD058003

PRIDE project URI

• PRIDE
  1. PXD058003
     1. Label: PRIDE project
     2. Name: Comparative single-cell and spatial transcriptomics illuminate bat immunity and barrier tissue evolution