PXD057969 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | KDM3A catalysed formation of hydroxyacetyl-lysine on histone H3K9 |
| Description | Histone modifications, including N-lysine acetylation and methylation, play critical roles in eukaryotic transcription. The addition of acetyl- and methyl-groups and removal of acetyl-groups involves redox neutral reactions, whereas demethylation is O2 dependent, a reaction with potential to enable O2 availability mediated regulation, as occurs for reactions catalysed by the 2-oxoglutarate dependent hypoxia-inducible factor (HIF) hydroxylases. A screen for substrates of the HIF-regulated Jumonji-C (JmjC) lysine demethylase KDM3A led to the finding that purified recombinant KDM3A catalyses oxidation of the N-acetyl group of Lys-9 of histone H3 giving an N-hydroxyacetyl-lysine residue. Studies employing a N-hydroxyacetyl-lysine selective antibody and mass spectrometry support the cellular relevance of N-hydroxyacetyl-lysine. The combined biochemical and cellular results reveal evidence for an unanticipated O2-mediated link between histone lysine N-acetylation and JmjC catalysis. Future work can explore the biological significance of N-hydroxyacetylation, including in the hypoxic response where KDM3A plays a role in regulating HIF target genes. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-10 |
| AnnouncementXML | Submission_2026-02-09_16:14:09.400.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Simone Sidoli |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | acetylated residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-11-17 12:00:19 | ID requested | |
| ⏵ 1 | 2026-02-09 16:14:09 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: histone demethylation / hydroxylation / acetylation / 2-oxoglutarate dependent oxygenase / JmjC KDMs / demethylases / KDM3A / KDM3B / hypoxia inducible factor / HIF prolyl hydroxylases / epigenetics |
Contact List
| Simone Sidoli |
|---|
| contact affiliation | Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA |
| contact email | simone.sidoli@einsteinmed.edu |
| lab head | |
| Simone Sidoli |
|---|
| contact affiliation | Albert Einstein College of Medicine |
| contact email | simone.sidoli@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD057969
- Label: PRIDE project
- Name: KDM3A catalysed formation of hydroxyacetyl-lysine on histone H3K9
