PXD057963-1

PXD057963 is an original dataset announced via ProteomeXchange.

Title	Mass Spectrometric Analysis of Clomiphene Citrate-induced Changes in the Secretome Profile of PAX8-positive Human Fallopian Tube Secretory Epithelial Cells and Identification of Biomarkers Relevant to Reproduction and Pregnancy
Description	Clomiphene citrate (CC) is commonly used for infertility treatments, particularly in intrauterine insemination (IUI) and in vitro fertilization (IVF) programs. Despite its effectiveness in inducing ovulation, it is reported to have a low pregnancy rate compared to letrozole. Further, risks of ectopic pregnancy, miscarriage, and fetal malformations have been reported in CC-induced fertility treatment cycles. The present study aimed at understanding whether these effects are associated with CC-induced changes in the secretome profile of the human PAX8-positive fallopian tube secretory epithelial cells (hPFTSECs). Fallopian tube tissues from healthy women who underwent postpartum tubectomy were enzymatically dissociated and cultured with CC, and their secretomes were analyzed by Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) to identify CC-induced alterations.
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-27_16:05:32.729.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Keshava Prasad T. S.
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-11-16 11:58:11	ID requested
⏵ 1	2025-07-27 16:05:33	announced

10.1007/s43032-025-01884-w;

Roy O, Ammankallu S, Narayana VK, Narayana C, Hebbar S, Adiga PK, Joshi MB, Gowda J, Kumar A, Adiga SK, Ritting M, Kannan N, Prasad TSK, Kalthur G, Mass Spectrometric Analysis of Clomiphene Citrate-induced Changes in the Secretome Profile of PAX8-positive Human Fallopian Tube Secretory Epithelial Cells and Identification of Biomarkers Relevant to Reproduction and Pregnancy. Reprod Sci, 32(7):2423-2441(2025) [pubmed]

submitter keyword: Infertility

Ovulation induction

Ectopic pregnancy

Selective estrogen receptor modulator (SERM)

Dr. T. S. Keshava Prasad
contact affiliation	Professor and Deputy Director
contact email	keshav@yenepoya.edu.in
lab head
Keshava Prasad T. S.
contact affiliation	Professor and Deputy Director, Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangalore, India
contact email	keshav@ibioinformatics.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD057963

PRIDE project URI

• PRIDE
  1. PXD057963
     1. Label: PRIDE project
     2. Name: Mass Spectrometric Analysis of Clomiphene Citrate-induced Changes in the Secretome Profile of PAX8-positive Human Fallopian Tube Secretory Epithelial Cells and Identification of Biomarkers Relevant to Reproduction and Pregnancy